Title:Dehydroabietylamine, A Diterpene from Carthamus tinctorious L. Showing Antibacterial and Anthelmintic Effects with Computational Evidence
Volume: 16
Issue: 3
Author(s): Aditya R. SJ, Ramesh CK, Raghavendra S and Paramesha M*
Affiliation:
- Molecular Biomedicine Laboratory and Department of P.G. Studies and Research in Biotechnology, Sahyadri Science College, Kuvempu University, Shivamogga, Karnataka,India
Keywords:
Carthamus tinctorius L., dehydroabietylamine, glucosamine-6-phosphate synthase, β-Tubulin, docking, helminth,
bactericidal.
Abstract:
Background: Plant-based drugs provide an outstanding contribution to modern therapeutics, and
it is well known that the presence of different phytochemicals is responsible for such pharmacological effects.
Carthamus tinctorius L. is one such medicinally important plant whose different solvent extracts
have been reported with several pharmacological effects like antibacterial, hepatoprotective, and wound
healing. The exploration of phytoconstituents from such a medicinally important plant for different pharmacological
effects could produce new and effective drugs to treat human diseases.
Objective: The present study attempts to explore the antibacterial and anthelmintic properties of
dehydroabietylamine, a diterpene isolated from Carthamus tinctorius L. followed by the in silico elucidation
of its probable mode of action.
Methods: The minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) of
dehydroabietylamine were assessed against Staphylococcus aureus and Pseudomonas aeruginosa, using micro-
broth dilution method. The anthelmintic activity of was determined to assess the time taken for paralysis
and death of Pheretima Posthuma at different concentrations. Additionally, molecular docking study was conducted
to understand the interaction between dehydroabietylamine with target proteins identified for both antibacterial
and anthelmintic activity viz., glucosamine-6-phosphate synthase and β-Tubulin, respectively.
Results: The dehydroabietylamine showed the significant MIC for S. aureus (12.5 μg/ml) and P. aeruginosa
(6.25μg/ml), respectively. The result of anthelmintics effect of dehydroabietylamine was found to be dosedependent
and compared to the standard drug, albendazole.
Conclusion: The interactions of dehydroabietylamine with the two target proteins with high binding affinity
indicated the probable inhibition of target proteins, which could be the cause for prominent antibacterial
and antihelminthic effects.
