Title:Human Leukocyte Antigens (HLA) Genes Association in Type 1 Diabetic Nephropathy
Volume: 19
Issue: 8
Author(s): Esma Mihoubi, Habiba Amroun, Rachida Raache*, Faiza Bouldjennet, Fethi Meçabih, Malha Azzouz, Aissa Boudiba, Souad Mahgoun, Malika Akachouche, Nawel Salhi, Chafia Touil-Boukoffa, Mohamed C. Abbadi and Nabila Attal
Affiliation:
- Laboratory of Cellular and Molecular Biology, Cytokine and NO Synthase Team University of Science and Technology, Houari Boumediene (USTHB), Algiers,Algeria
Keywords:
Type 1diabetes, diabetic nephropathy, class I HLA genes, class II HLA genes, alleles, analysis, haplotype analysis.
Abstract:
Background: Diabetic nephropathy is a common worldwide multifactorial disease where
involvement of genetic factors is well etablished. The aim of this study was to investigate the HLA
genes implication in the development of type 1 diabetic nephropathy.
Methods: We performed a case- control study where one hundred and fifty subjects were examined.
Patients were divided in two groups; with and without type 1 diabetic nephropathy. HLA typing was
performed using Polymerase Chain Reaction- Sequence Specific Oligonucleotide (PCR- SSO) method.
HLA association to clinical phenotype and HLA haplotype analysis was also investigated.
Results: HLA B*51 is increased in patients without type 1 diabetic nephropathy (7.14% vs. 0 %, P <0.05,
OR= 0), however no other studied alleles seem to have any effect (all P>0.05). Haplotype analysis also
does not reveal any significant association, however, A*02-B*18-DRB1*03-DQA1*05- DQB1*03 haplotype
shows a tendency to be associated with the development of diabetic nephropathy (P = 0.05).
Conclusion: These results suggest a protective effect of HLA B*51 allele from type 1 diabetic nephropathy.
However, further studies are required in order to clarify its potential implication as a protective
marker.