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Review Article

生物样品中植物甾醇测量的方法学方面

卷 26, 期 37, 2019

页: [6776 - 6785] 页: 10

弟呕挨: 10.2174/0929867325666180713160330

价格: $65

摘要

在过去的二十年中，在食品和添加了4-去甲基植物甾醇的补充剂被公认为有效且安全的非药物降胆固醇药之后，以及在肠道吸收和甾醇经肝排泄的机制之后，植物甾醇的测量引起了广泛的关注。被解散了。另外，血清植物甾醇作为胆固醇吸收的生物标记物的广泛使用增加了对其测量的兴趣。在这篇综述中，讨论了基本方法，而没有详细介绍实际操作。分析包括首先从各种生物基质（例如血清/血浆，粪便或组织）中提取脂质并进行皂化，然后根据吸附剂的极性通过吸附色谱法（气液或液体或高效液相色谱法）分离出单独的固醇。各种固醇。我们还处理生物样品中植物甾醇测量的某些特定方面，例如需要统一其在生物样品中的分析，不同研究之间谷固醇和菜油甾醇浓度的差异以及对它们的生物学天知日波动。植物固醇在人类健康中具有重要作用，因此其复杂而耗时的测量方法引起人们对固醇研究实验室之间标准化的常规方法的关注。

关键词: 金刚甾醇，菜油甾醇，菜油甾醇，胆固醇，气液色谱法，谷甾醇，谷甾烷醇，豆甾醇。

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