Title:Anticancer Activity Study of Chromone and Coumarin Hybrids using Electrical Impedance Spectroscopy
Volume: 18
Issue: 6
Author(s): Mustapha M. Bouhenna, Nabil Mameri*, Mónica V. Pérez, Oualid Talhi, Khaldoun Bachari, Artur M.S. Silva and Walter Luyten
Affiliation:
- Unite de Recherche Engineering Environnement (URIE), Ecole Nationale Polytechnique, 10 Avenue Pasteur, El Harrach, Alger,Algeria
Keywords:
Coumarin, chromone, impedance, cell sine, anticancer, cytotoxicity.
Abstract: Aims: Oncology treatments aim at selective toxicity for tumor (compared to normal) cells, and chromone-
coumarin hybrids have shown such activity.
Methods: In this study, we test a novel series of synthetic chromone and coumarin derivatives (1–9) for cytotoxic
activity against a panel of tumor cell lines (MCF-7, A549, HepG2, HTC-116, B16 and Caco-2) opposed to
non-tumor cells (HEK-293t). Electrical impedance spectroscopy was used to monitor cell viability in real time.
Results: Compound 8 showed the most potent activity, and it significantly diminished cancer cell proliferation
and viability in different cell lines. It induced apoptosis in a dose-dependent manner, as shown by Western blot
and flow cytometry.
Conclusion: Electrical impedance spectroscopy appears to be a convenient tool for in vitro cytotoxicity analysis,
which could be useful for identifying drug effects and side effects during early phases of drug discovery and
development.
