Title:Neuroglial Cell Cryopreservation in Appropriate Inductive Conditions
Volume: 23
Issue: 28
Author(s): Rosa M. Gomez*, Maria Andrea Dominguez-Sanchez, Magdy Y. Sanchez and George E. Barreto
Affiliation:
- Neuroregeneration Foundation of Colombia. NeuroRec Research Group., Bogota,Colombia
Keywords:
Cryopreservation, cell therapy, stem cell, neuroglia cell, cryoprotectant, neuroregeneration.
Abstract: An ability to store cells (if they cannot be used fresh) reduces cell wastage, thereby increasing the
supply of transplantable material. Cell storage is also valuable in scientific research, allowing material to be archived
and experiments to be repeated from the same tissue source and facilitating research collaboration by
allowing stored samples to be distributed. Cryopreservation is currently considered the most promising and successful,
long-term biological conservation method. Its use has led to optimizing survival, improving protocols and
stem cell and neuroglia viability, thereby assuring its future use in neuroregeneration and restoration regarding
cell therapy. The success of conservation processes in ensuring cell viability depends on aspects such as the characteristics,
cells' cryobiological behavior, isolation methodologies, cell freezing, the use and choice of cryoprotectants
and such aspects' influence on intra- and extra-cellular dynamics. This review deals with cells' cryobiological
behavior, cryopreservation and cryoprotectants, emphasizing on stem cell and neuroglial populations as
therapeutic target regarding nervous system diseases.