Title:Wrap-and-Strip Technology of Protein–Polyelectrolyte Complex for Biomedical Application
Volume: 23
Issue: 3
Author(s): Kentaro Shiraki, Takaaki Kurinomaru and Shunsuke Tomita
Affiliation:
关键词:
抗体，酶，富集，聚电解质，酶活力，聚集，沉淀。
摘要: A polyelectrolyte is a polymer composed of repeating units of an electrolyte group that enables reversible
complex formation with proteins in aqueous solutions. This review introduces “wrap-and-strip” technology
of protein–polyelectrolyte complex (PPC) by noncovalent interaction. Storage: protein is stabilized
against physical and chemical stresses. Enrichment: precipitation through PPC can be used as an enrichment
method without irreversible unfolding. Catalytic activity switch: a complementary charged pair of polyelectrolytes
functions as a reversible enzyme activity switch. Hyperactivation: a specific combination of a polyelectrolyte
and substrate enhances enzyme activity by one order of magnitude compared with an enzyme alone.
Stabilization: PPC increases protein stability against chemical and physical stresses, such as covalently modified
polyethylene glycosylated protein. Simple PPC-based technology can expand the applicable fields of
soluble proteins in aqueous solutions.
