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Abstract
A polyelectrolyte is a polymer composed of repeating units of an electrolyte group that enables reversible complex formation with proteins in aqueous solutions. This review introduces “wrap-and-strip” technology of protein–polyelectrolyte complex (PPC) by noncovalent interaction. Storage: protein is stabilized against physical and chemical stresses. Enrichment: precipitation through PPC can be used as an enrichment method without irreversible unfolding. Catalytic activity switch: a complementary charged pair of polyelectrolytes functions as a reversible enzyme activity switch. Hyperactivation: a specific combination of a polyelectrolyte and substrate enhances enzyme activity by one order of magnitude compared with an enzyme alone. Stabilization: PPC increases protein stability against chemical and physical stresses, such as covalently modified polyethylene glycosylated protein. Simple PPC-based technology can expand the applicable fields of soluble proteins in aqueous solutions.
Keywords: Antibody, enzyme, enrichment, polyelectrolyte, enzyme activity, aggregation, precipitation.
Current Medicinal Chemistry
Title:Wrap-and-Strip Technology of Protein–Polyelectrolyte Complex for Biomedical Application
Volume: 23 Issue: 3
Author(s): Kentaro Shiraki, Takaaki Kurinomaru and Shunsuke Tomita
Affiliation:
Keywords: Antibody, enzyme, enrichment, polyelectrolyte, enzyme activity, aggregation, precipitation.
Abstract: A polyelectrolyte is a polymer composed of repeating units of an electrolyte group that enables reversible complex formation with proteins in aqueous solutions. This review introduces “wrap-and-strip” technology of protein–polyelectrolyte complex (PPC) by noncovalent interaction. Storage: protein is stabilized against physical and chemical stresses. Enrichment: precipitation through PPC can be used as an enrichment method without irreversible unfolding. Catalytic activity switch: a complementary charged pair of polyelectrolytes functions as a reversible enzyme activity switch. Hyperactivation: a specific combination of a polyelectrolyte and substrate enhances enzyme activity by one order of magnitude compared with an enzyme alone. Stabilization: PPC increases protein stability against chemical and physical stresses, such as covalently modified polyethylene glycosylated protein. Simple PPC-based technology can expand the applicable fields of soluble proteins in aqueous solutions.
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Cite this article as:
Shiraki Kentaro, Kurinomaru Takaaki and Tomita Shunsuke, Wrap-and-Strip Technology of Protein–Polyelectrolyte Complex for Biomedical Application, Current Medicinal Chemistry 2016; 23 (3) . https://dx.doi.org/10.2174/0929867323666151127201126
DOI https://dx.doi.org/10.2174/0929867323666151127201126 |
Print ISSN 0929-8673 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-533X |
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