Title:1, 3, 6-Trihydroxy-7-Methyl-9, 10-Anthracenedione Isolated from genus Lindera with Anti-Cancer Activity
Volume: 17
Issue: 11
Author(s): Guoqing Wei, Hui Chen, Fangyuan Nie, Xiaofeng Ma*Hezhong Jiang*
Affiliation:
- College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049,China
- School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031,China
Keywords:
Anthraquinone, breast cancer, fatty acid synthase, inhibit, Lindera aggregate, Lindera nacusua, 1, 3, 6-Trihydroxy-7-methyl-9, 10-
anthracenedione (TMA).
Abstract: Background: Natural products inhibiting fatty acid synthase are potential therapeutic agents to treat
cancer.
Objective: To investigate the chemical constituents of the root tubers of Lindera aggregate and the stems of
Linderanacusua, and to find natural inhibitors on the expression level on fatty acid synthase in human breast
cancer MDA-MB-231 cells.
Methods: The isolation and purification of the extracts were conducted by the methods of percolation and
partition extraction, silica and gel column chromatography, recrystallization and by NMR and spectroscopic
analysis method. The cell viability was assessed by Cell Counting Kit assay.
Results: Seven compounds were elucidated, mainly including five sesquiterpenes, one anthraquinoe and
one γ -butanolide, in which compounds 6 was firstly reported from genus Lindera, and compound 7 was
isolated from Linderanacusuafor the first time. Among them, 1,3,6-Trihydroxy-7-methyl-9,10-anthracenedione
(TMA, compound 6) showed strong inhibitory effect on the expression level on fatty acid synthase in human
breast cancer MDA-MB-231 cells. In addition, TMA was found to reduce breast cancer cells viability dosedependently.
Conclusion: The fatty acid synthase was a potent therapeutic target for cancers, these findings suggest that TMA
has the application potential for treating human cancers.
