Title:Normobaric Hyperoxia Preconditioning Induces Changes in the Brain Lipidome
Volume: 12
Issue: 4
Author(s): Masoumeh Faezi, Mohammad Reza Bigdeli, Fateme Mirzajani and Alireza Ghasempour
Affiliation:
Keywords:
Brain ischemia, Lipidome, Neuroprotection, Normobaric hyperoxia, Preconditioning, Stroke.
Abstract: Recent investigations have demonstrated that normobaric hyperoxia induces neuroprotection against ischemic
injury. The aim of study was to determine the survey of HO (hyperoxia) preconditioning on brain lipidome.The animals
were assigned into three groups, the first experimental group was exposed to 95% inspired HO for 4 h /day for six consecutive
days. The second experimental group considered as the control group and was exposed to 21% oxygen as room
air (RA) in the same chamber. The third group acted as sham, which was under the stress of surgery condition without
ischemia. The first two groups were divided into 2 subgroups, intact (without any surgery) and middle cerebral artery occlusion-
operated (MCAO). Twenty-four hours after exposure to hyperoxia, MCAO subgroups were subjected to 60 min
of right middle cerebral artery occlussion. After 24 h reperfusion, infarct volume (IV) and neurological deficit score
(NDS) were assessed in MCAO subgroup. Brain lipidomics were measured in the intact subgroup. Preconditioning with
HO significantly reduced NDS and IV and elevated the level of phosphatidylethanolamine (PE), sphingomyelin (SM),
cholesterol ester (CE), cholesterol (Chol), phosphatidylcholine (PC), triglyceride (TG) and cerebroside (CB) in the brain
as compared with the control (sham and RA). HO preconditioning, significantly decreased the brain ceramide (Cer) and
lyso- phosphatidylcholine (Lyso-PC or LPC) levels. Preconditioning with HO decreases brain ischemia injury via changes
in brain lipidomics and significantly decreases the brain ceramides (CER).Although more studies are required to explain
the mechanisms of time course of neuroprotection, HO preconditioning partly decreases brain ischemia injury via changes
in brain lipidome.