Title:Identification of Target Antigens of Antiendothelial Cell Antibodies Against Human Brain Microvascular Endothelial Cells in Healthy Subjects
Volume: 12
Issue: 1
Author(s): Akio Kimura, Takeo Sakurai, Nobuaki Yoshikura, Akihiro Koumura, Yuichi Hayashi, Hirofumi Ohtaki, Mitsuhiro Chousa, Mitsuru Seishima and Takashi Inuzuka
Affiliation:
Keywords:
Autoantibody, blood-brain barrier, immunology, proteomics.
Abstract: Antiendothelial cell antibodies (AECAs) have been detected in patients who have autoimmune
and inflammatory diseases. Previous studies showed that AECAs against human umbilical vein endothelial
cells were detected in healthy subjects. In the present study, we evaluated AECAs against human brain
microvascular endothelial cells (HBMEC) in serum. We detected 250 antigen spots that reacted with
AECAs in serum samples from 30 healthy subjects by 2-dimensional immunoblot using primary cultured
HBMEC as the antigen source. There were 10 spots that corresponded to common target antigen spots and
reacted with AECAs in serum samples from > 25% of the 30 healthy subjects. We identified 7 proteins that corresponded
to 8 of the 10 spots by mass spectrometry: 78-kDa glucose-regulated protein, dihydropyrimidinase-related protein 2,
heterogeneous nuclear ribonucleoprotein L, vimentin, perilipin 3, alpha-enolase, and annexin A2. The results suggest that
these 7 HBMEC proteins are major target antigens of natural AECAs.
