Title:Synthesis of Six Phenylalanine Derivatives and their Cell Toxicity Effect on Human Colon Cancer Cell Line HT-29
Volume: 12
Issue: 6
Author(s): Qianyi Zhao, Ting Xu, Menghua Li, Ying Yang, Haopeng Hu, Shupei Wang, Wenjuan Yan, Ran Chen, Chunyan Zhang and Cunshuan Xu
Affiliation:
Keywords:
Phenylalanine, L-4-phenylalanine derivatives, human colon cancer cell line HT-29 (HT-29 cells), median lethal
dose (LD50), cell toxicity, cell apoptosis.
Abstract: Some phenylalanine (Phe) derivatives had important roles in pharmacology and may be
used as pharmaceutical materials and pharmaceutical intermediates. In order to understand the cell
toxicity of phenylalanine derivatives, we synthesized L-4-bromo-phenylalanine (Brp), L-4-iodophenylalanine
(Ip), L-4-nitro-phenylalanine (Np), L-4-sulfonic-phenylalanine (Sp), L-4-phosphophenylalanine
(Pp) and L-4-amino-phenylalanine (Np). We used mass spectrometry (MS), Infrared Spectroscopy (IR) or
hydrogen-1 nuclear magnetic resonance spectrum (1H-NMR), and high performance liquid chromatography (HPLC) to
test the correction of these products, MTT assay and Hoechst33258 staining to detect their cell toxic effect on human colon
cancer cell HT-29 (HT-29 cells). The results showed that these products were correct and the cytotoxicity of
PpIpSp and NpBrp, Ap almost had no effect on HT-29 cells. In addition, Pp, Ip and Sp induced cell apoptosis, the
other three kinds of phenylalanine derivatives induced neither apoptosis nor necrosis.