Title:Proteomic-based Analysis for Identification of Proteins Involved in 5-fluorouracil Resistance in Hepatocellular Carcinoma
Volume: 20
Issue: 1
Author(s): Yi Tan, Shukui Qin, Xin Hou, Xiujuan Qian, Jun Xia, Yumei Li, Rui Wang, Changjie Chen, Qingling Yang, Lucio Miele, Qiong Wu and Zhiwei Wang
Affiliation:
Keywords:
Hepatocellular carcinoma, chemoresistance, 5-FU, PRDX6, PMSB7.
Abstract: Background: Hepatocellular carcinoma (HCC) has high mortality partly due to acquiring drug resistance during chemotherapy
treatment. Therefore, it is necessary to explore the underlying mechanism of drug resistance.
Methods: We used 2-DE and MALDI-TOF-MS analysis to explore the possible molecular insight into 5-FU resistance in HCC. The differentially
expressed proteins were validated by Western blot analysis.
Results: We identified 102 unique proteins including p16, maspin, PRDX6, PSMB7, MYL6, PHB, and HSP27 with alteration in SMMC-
7721/5-FU. Furthermore, down-regulation of PRDX6 and PSMB7 enhanced SMMC-7721/5-FU cells to 5-FU sensitivity.
Conclusions: Our study suggests that targeting drug resistant genes such as PRDX6 and PSMB7 could be a novel approach to overcome
5-FU resistance in HCC cells.
