Title:Characterization of Lin-ve CD34 and CD117 Cell Population Reveals an Increased Expression in Bone Marrow Derived Stem Cells
Volume: 11
Issue: 1
Author(s): Neeru Jindal, Gillipsie Minhas, Sudesh Prabhakar and Akshay Anand
Affiliation:
Keywords:
Bone marrow, CD34, CD45, CD117, EPCs, Sca1, Stem cell.
Abstract: The purpose of the study was to evaluate the expression of CD45, CD34, Sca-1 and CD117 in mouse bone
marrow, Lin-ve and Lin+ve population. Bone marrow cells were isolated from C57/BL6J mouse and mononuclear
population was separated from rest of the cell population. With the help of Magnetic associated cell sorter (MACS), Linve
and Lin+ve cells were separated from the bone marrow. The expression of CD45, CD34, Sca1 and CD117 was
evaluated in bone marrow, Lin-ve and Lin+ve population by flow cytometry. We found a significant increase in the
expression of CD34 and CD117 in Lin-ve as compared to the bone marrow and Lin+ve population. These findings
suggest that Lin-ve population has higher expression of stem cell progenitor markers and could be useful for tissue repair
and regeneration.
