Title: New Indocyanine Derivatives for the Synthesis of Fluorescently Labeled Oligonucleotides
Volume: 6
Issue: 1
Author(s): Edward N. Timofeev, Victoria E. Kuznetsova, Alexander S. Zasedatelev and Alexander V. Chudinov
Affiliation:
Keywords:
Oligonucleotides, fluorescent label, phosphoramidites, controlled pore glass, indocyanines, deprotection
Abstract: New sulfonated Cy3 and Cy5 phosphoramidites and solid supports were synthesized. These building blocks were used in the synthesis of terminally or internally labeled oligodeoxynucleotides. Mild deprotection schemes were applied to the sulfocyanine-labeled oligonucleotides.