Title:Effect of Raltitrexed on ECA109 Cellular Radiosensitivity and its Mechanism
in Esophageal Cancer
Volume: 30
Issue: 19
Author(s): Li-Ben Xu, Chao-Yang Wu, Yan Wang and Ju-Ying Zhou*
Affiliation:
- Department of Radiotherapy, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu, China
Keywords:
Raltitrexed, radiosensitization, esophageal cancer, migration and invasion, apoptosis, Bax/Bcl-2 pathway.
Abstract:
Background: To investigate the effect of raltitrexed + X-ray irradiation on esophageal cancer
ECA109 cells and analyze the potential action mechanism.
Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to analyze
the inhibitory effect of raltitrexed on cell proliferation. The effect of raltitrexed on radiosensitivity was
studied through a clone-forming experiment. The scratch assay and invasion test were performed to understand
the cell migration and invasion abilities. The apoptosis rate change was measured using a flow cytometer,
and Western Blotting was used to determine the expression of B cell lymphoma-2 (Bcl-2) and Bcl2-associated
X protein (Bax) in each group.
Results: Raltitrexed significantly inhibited ECA109 proliferation in a time-dose-dependent manner; there
were significant differences among different concentrations and times of action. The results of the clone-forming
experiment showed a sensitization enhancement ratio of 1.65, and this demonstrated a radiosensitization effect.
After the combination of raltitrexed with X-ray, the cell migration distance was shortened, and the number
of cells penetrating the membrane was reduced.
Conclusion: Raltitrexed can inhibit the growth of esophageal cancer ECA109 cells and has a radiosensitization
effect.
