Title:Anti-tumoral Immunity and Chemo-preventive Effectiveness of Herbal Extracts of
Curcumin, Ginger, Clove and Amygdaline in Ehrlich Ascites Carcinoma-Challenging
Mice
Volume: 24
Issue: 11
Author(s): Soha Gomaa*, Mohamed Nassef, Randa El-Naggar, Ahmed Massoud and Mona El-Kholy
Affiliation:
- Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt
Keywords:
Herbal extracts, chemo-prevention, anti-tumor immunity, apoptosis, cancer, conventional chemotherapy.
Abstract:
Background: Due to its systemic toxicity, traditional chemotherapy of tumors is being taken into consideration.
Herbal therapy, containing phytochemical polyphenol derivatives such as Curcumin (Cur), Ginger
(Gin), Cloves (Clov) and Amygdaline (Amyg), is one of the numerous complementary and alternative approaches
as an anti-cancer therapy and holds great promise for cancer chemo-prevention with fewer side effects.
Aim: The current study was designated to assess anti-tumoral immunity and anti-cancer and chemo-preventive effectiveness
of herbal extracts of Cur, Ginger, Clov and Amyg in Ehrlich Ascites Carcinoma (EAC)-challenging mice.
Methods: Chemo-preventive efficacy of herbal extracts of Cur, Gin, Clov and Amyg were analyzed in vivo by
examination of the apoptosis rate of EAC tumor cells by flow cytometry. The total numbers of EAC cells, splenocytes
counts and leucocytes count with their differentials relative % in peripheral blood (PB) of EACchallenging
mice were investigated.
Results: EAC-challenging mice treated with herbal extracts of Cur, Gin, Clov and Amyg showed a marked decline
in EAC tumor cell count and a noticeable increase in apoptosis rate of EAC tumor cells, a remarkable decrease
in serum level of cancer antigen 125 (CA-125) with an obvious increase in the number of splenocytes
comparing to that in EAC-challenging mice treated with PBS alone. Moreover, the data indicated an insignificant
change in the total leucocytes count and their differentials relative % of eosinophil, neutrophils, monocytes and
lymphocytes in EAC-challenging mice treated with Cur and Amyg, but these parameters were markedly increased
in EAC-challenging mice injected with Gin and Clov compared to that in EAC-challenging mice treated
with PBS alone.
Conclusion: To conclude, the herbal extracts of Cur, Gin, Clov and Amyg may have anti-tumoral immunity and
anti-cancer potency and potential to reduce the resistance to cancer conventional chemotherapy and exert cancer
chemo-protective approaches with low adverse effects. Further research is necessary to determine the regimen's
toxicity on various tissues and organs and to connect the diagnostic and therapeutic approaches used in the regimen's
biomedical use.
