Title:Antioxidant Activities and Phenolic Composition of Sarcococca saligna
Leaves
Volume: 21
Issue: 10
Author(s): Syed Mubashar Sabir, Shabnam Shahida, Alam Zeb, Syed Rizwan Abbas, Muhammad Usman Hameed, Abdallah Shanableh, Muhammad Imran Khan, Djamel Ghernaout, Ramzi Hadj Lajimi, Sarra Elgharbi, Malek Besbes and Noureddine Elboughdiri*
Affiliation:
- Department of Chemical Engineering, College of Engineering, University of
Hail, PO Box 2440, Ha’il 81441, Saudi Arabia
Keywords:
Sarcococca saligna, DPPH activity, lipid peroxidation, metal chelation, HPLC analysis, antioxidants.
Abstract: Sarcococca saligna (D. DON) Muel is a vital plant with several steroidal alkaloids and is
effective against syphilis, liver diseases, gastrointestinal tract disease, infections, and inflammation.
The present study aimed to investigate the in vitro antioxidant activities and phenolic profile of S.
saligna. High-performance liquid chromatography (HPLC) analysis has shown the presence of
twelve phenolics namely kaempferol-3-O-glucoside, proanthocyanidin B1, quercetin-3-malonylglucoside-
7-glucoside, kaempferol-3-O-sophorotioside, Isorhamnetin-3-O-glucoside-7-O-rhamnoside,
kaempferolhexoside, 4-O-Caffeoyl-5-O-p-coumaroylquinic acid, 3,4-Di-O-caffeoyl-5-O-feruloylquinic
acid, 3,4-Di-O-feruloyl-5-O-caffeoylquinic acid, flavogalloyl-HHDP-gluconic acid (lagerstannin B),
3,4-di-O-caffeoylquinic acid and kaempferol-3-(caffeoyldiglucoside)-7-glucoside. The hot water
extract showed the highest anti-lipid peroxidative activities. The IC50 value for 2,2-diphenyl-1-
picrylhydrazyl (DPPH) radical scavenging activity was 71.5 ± 2.1 μg/mL. The extract could chelate
the iron and possess reducing activities on phosphomolybenum assay. It is concluded that extracts of
S. saligna are rich in antioxidants and contain essential phytochemicals.
