Title:Preparation Optimization and Immunological Activity Studies of Portulaca oleracea
L. Polysaccharides Liposomes
Volume: 30
Issue: 10
Author(s): Yan Li, Guiyan Jia, Tao Li, Xiechen Zhou, Hui Zhao, Junyang Cao, Zijan Guan and Rui Zhao*
Affiliation:
- College of Life Science & Biotechnology, Heilongjiang Bayi Agricultural University, Daqing High-Tech Industrial Development
Zone, Daqing, Heilongjiang Province 163319, P.R. China;
Keywords:
Portulaca oleracea L. polysaccharides, liposomes, reverse evaporating method, process optimization, immunoenhancing activity, response surface methodology.
Abstract:
Aims: This study combines traditional Chinese medicine polysaccharides with nanomaterials to
enhance drug bioavailability and immunological activity.
Background: The study of polysaccharide preparation, structure identification, pharmacological activity,
and mechanism of action is deepening, but the research combined with the new drug delivery system is relatively
weak, so the application of polysaccharides is still facing great limitations. In order to prolong the
action time of polysaccharides and improve their bioavailability, liposome has become the most promising
delivery carrier.
Objectives: The purpose of this study was to optimize the preparation process of Portulaca oleracea L. polysaccharides
liposomes (POL-PL) and evaluate the immunoactivity in vitro.
Methods: POL-PL was prepared by reverse evaporation, and the preparation process was optimized using the
response surface methodology. The characteristic analysis of POL-PL was detected by the indicators including
morphology, particle size, zeta potential, encapsulation efficiency, release, and stability. The effects of
POL-PL on the proliferation and immunological activity of mouse spleen lymphocytes and RAW264.7 cells
were evaluated in vitro.
Results: POL-PL is highly homogeneous in morphology and particle size, and its sustained release improves
the bioavailability of Portulaca oleracea L. polysaccharides (POL-P). Moreover, POL-PL treatment significantly
enhanced the proliferation and phagocytic activity of RAW264.7 cells and increased the secretion of
IL-6, TNF-α, IL-1β, and NO.
Conclusion: This study suggested that POL-PL were prepared successfully by reverse evaporation method,
and POL-PL had immunoenhancing activity in vitro. The results provided a theoretical basis for further application
of POL-PL.
