Title:Genetic Polymorphisms of GP1BA, PEAR1, and PAI-1 may be Associated
with Serum sIgE and Blood Eosinophil Levels in Chinese Patients with
Allergic Diseases
Volume: 24
Issue: 10
Author(s): Rui Tang, Xiaohong Lyu, Jinlyu Sun*Hong Li*
Affiliation:
- State Key Laboratory of Complex Severe and Rare Diseases, Allergy Department, Peking Union Medical College
Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
- Beijing Key Laboratory
of Precision Medicine for Diagnosis and Treatment of Allergic Diseases, Allergy Department, National Clinical
Research Center for Dermatologic and Immunologic Diseases, Peking Union Medical College Hospital,
Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
- State Key Laboratory of Complex Severe and Rare Diseases, Allergy Department, Peking Union Medical College
Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
- Beijing Key Laboratory
of Precision Medicine for Diagnosis and Treatment of Allergic Diseases, Allergy Department, National Clinical
Research Center for Dermatologic and Immunologic Diseases, Peking Union Medical College Hospital,
Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
Keywords:
Allergy and immunology, gene polymorphism, platelet endothelial aggregation receptor 1 (PEAR1), specific immunoglobulin E (sIgE), eosinophil, glycoprotein Ib alpha gene (GP1BA), plasminogen activator inhibitor 1 gene (PAI-1).
Abstract:
Background: It has been suggested that genetic factors may be substantially linked to
allergy disorders.
Objective: This study aims to investigate the relationship between the serum specific Immunoglobulin
E (sIgE), blood eosinophil, and the polymorphisms of glycoprotein Ib alpha gene
(GP1BA) rs6065, platelet endothelial aggregation receptor 1 gene (PEAR1) rs12041331, and
plasminogen activator inhibitor 1 gene (PAI-1) rs1799762.
Methods: From the Peking Union Medical College Hospital, this study enrolled 60 healthy participants
and 283 participants with allergic diseases. TaqMan-minor groove binder (MGB)
quantitative polymerase chain reaction (qPCR) was used to examine the gene polymorphisms in
each group.
Results: The TaqMan-MGB qPCR results were completely consistent with the DNA sequencing
results, according to other studies in this medical center (Kappa =1, p <0.001). The GP1BA
rs6065, PEAR1 rs12041331, and PAI-1 rs1799762 polymorphisms did not show different distribution
between allergy patients and healthy individuals. Concerning allergy patients, the CT
(n=33) genotype of GP1BA rs6065 had higher blood eosinophil level than the CC (n=250)
genotype (0.59, IQR 0.32-0.72 vs 0.31, IQR 0.15-0.61, *109/L, p =0.005). The serum sIgE of
AA (n=46) genotype of PEAR1 rs12041331 was lower (median 3.7, interquartile quartiles
(IQR) 0.2-16.8, kU/L) than the GA (n=136) and GG (n=101) genotypes (GA median 16.3, IQR
3.1-46.3, kU/L, p = 0.002; GG median 12.9, IQR 3.0-46.9, kU/L, p =0.003). The GA genotypes
of PEAR1 rs12041331were with higher blood eosinophil levels (median 0.42, IQR 0.17-0.74
*109/L) than the AA genotype (median 0.25, IQR 0.15-0.41*109/L, p =0.012). The sIgE of the
5G5G (n=44) genotype of PAI-1 rs1799762 was lower (median 5.0, IQR 0.1-22.8, kU/L) than
the 4G5G (n=144) (median 17.3, IQR 3.7-46.0, kU/L, p = 0.012).
Conclusion: The GP1BA rs6065, PEAR1 rs12041331, and PAI-1 rs1799762 polymorphisms
may be associated with the genetic susceptibility of serum sIgE or blood eosinophil in Chinese
allergic disease patients.
