Title:Integrated Bioinformatics Analysis and Target Drug Prediction of Inflammatory Bowel Disease Co-existent Diabetes Mellitus
Volume: 21
Issue: 2
Author(s): Lili Yang, Ning Wang, Yutong Wang, Wen Li, Ziyang Kong, Bin Zhang*Yaoyao Bian*
Affiliation:
- Department of Gastroenterology, Ningbo Municipal
Hospital of TCM, Affiliated Hospital of Zhejiang Chinese Medical University, Ningbo, 315012, China
- Jiangsu Provincial Engineering
Center of TCM External Medication Researching and Industrializing, Nanjing University of Chinese Medicine, Nanjing,
210023, China
- School of Acupuncture-Moxibustion and Tuina, School of Health Preservation and Rehabilitation,
Nanjing University of Chinese Medicine, Nanjing, 210023, China
Keywords:
Inflammatory bowel disease, diabetes mellitus, integrated bioinformatics analysis, targeted drug prediction, differentially expressed genes, gene expression omnibus public database.
Abstract:
Introduction: Inflammatory bowel disease (IBD) has become one of the public problems
worldwide and its incidence rate is increasing year by year. Its concomitant disease i.e.
diabetes mellitus (DM) has attracted more and more attention due to DM altering the progression
of IBD and leading to long periods of intermittent recurrence and deterioration. The common
mechanism and potential target drug of IBD with comorbid chronic conditions of DM were
explored.
Methods: Gene expression profile data were downloaded from the Gene Expression Omnibus
(GEO) public database. The differentially expressed genes (DEGs) were identified by R software.
GO annotation and pathway enrichment were performed, a protein-protein interaction
(PPI) network was constructed, associated lncRNAs were predicted and drug prediction targeting
key genes was made. Additionally, the regulatory network among core genes, associated
pathways, and predicted lncRNA in IBD with coexistent DM were visualized.
Results: We identified the critical gene MMP3 with lncRNA CDKN2BAS involved in the
PPAR pathway, which uncovered the underlying regulatory mechanism of IBD with coexistent
DM. We also predicted the potential therapeutic compound ZINC05905909 acting on MMP3.
Conclusion: Our findings revealed the regulatory mechanism chain of critical gene MMP3,
lncRNA CDKN2BAS, and PPAR pathway and provided potential therapeutic compound
ZINC05905909 for drug therapy to treat comorbid IBD DM.
