Title:Identification of an Exosome-relevant SNHG6-hsa-miR-429-
CHRDL1/CCNA2 Axis for Lung Adenocarcinoma Prognosis
Evaluation
Volume: 31
Issue: 28
Author(s): Yuan Yuan, Qian Li, Feifei Chen, Yujie Zhao, Jiyong Ma*Surong Fang*
Affiliation:
- Department of Respiration, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, China
- Department of Respiration, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, China
Keywords:
Lung adenocarcinoma, exosome, ceRNAs, hsa-miR-429, CHRDL1, CCNA2.
Abstract:
Aim: To explore an exosome-relevant molecular classification in lung adenocarcinoma
(LUAD).
Background: Exosome genes or relevant non-coding RNAs are regulators of cancer
treatment and prognosis, but their function in LUAD has not yet been determined.
Objective: Unraveling a molecular classification applying exosome-related RNA networks
for LUAD prognosis evaluation.
Methods: MicroRNA sequencing data (miRNAs-seq) and RNA sequencing data (RNA-
seq) were derived from The Cancer Genome Atlas (TCGA). The ConsensusCluster-
Plus package was used for molecular typing in LUAD based on 121 Exosome-related
genes. Then, a limma package was conducted to explore differentially expressed mRNAs
(DEmRNAs), differentially expressed miRNAs (DEmiRNAs) and differentially expressed
lncRNAs (DElncRNAs) in molecular typing for constructing an Exosome-driven
competing endogenous RNA network (ceRNA). Dominant miRNAs, as well as target
mRNAs, were identified by COX modeling and Kaplan-Meier survival analysis.
Results: Two Exosome-associated molecular clusters classified in LUAD. The C2 cluster
favored high clinicopathology and showed a trend toward poor prognosis. 29 lncRNA-
miRNA and 12 miRNA-mRNA interaction pairs were identified. The hsa-miR-429
was the pivotal miRNA in the network that affected the prognosis of LUAD. According
to the interaction relationship and LUAD prognostic role, SNHG6-hsa-
miR-429-CHRDL1/CCNA2 was identified. SNHG6-hsa-miR-429-CHRDL1 exerts
oncogenic effects, and SNHG6-hsa-miR-429- CCNA2 exerts pro-oncogenic effects.
Conclusion: Overall, our study identified an Exosome-driven ceRNA network in LUAD,
and the SNHG6-hsa-miR-429-CHRDL1/CCNA2 axis could be a new therapeutic
target for LUAD and our study provides new insights into the molecular mechanisms of
LUAD.
