Title:Preparation of Lycium barbarum Active Glycopeptide and Investigation of Its
Apoptotic Effects on Melanoma
Volume: 24
Issue: 2
Author(s): Jinghua Qi, Xingli Qi, Hongyuan Chen*Wen Rui*
Affiliation:
- Department of Pathogenic Biology and Immunology, School of Life Sciences and Biopharmaceuticals, Guangdong
Pharmaceutical University, Guangzhou, 510006, Guangdong Province, P.R. China
- Guangdong Cosmetics Engineering & Technology
Research Center, Guangzhou, 510006, Guangdong Province, P.R. China
- Centre for Novel Drug Research and Development, Guangdong Pharmaceutical University, Guangzhou, 510006, Guangdong Province,
P.R. China
- Key Laboratory of Digital Quality Evaluation of Chinese
Materia Medica of State Administration of TCM, Guangdong Pharmaceutical University, Guangzhou, 510006, Guangdong
Province, P.R. China
Keywords:
Apoptosis, glycopeptide, Lycium barbarum, melanoma, P38-MAPK/AKT, polysaccharides.
Abstract:
Introduction: The increasing number of studies have shown that Lycium barbarum polysaccharides
possess anti-tumor effects. However, the determination of the active ingredients and their mechanism against
melanoma inhibition are still unknown.
Methods: In this study, we aimed to investigate the mechanisms of action of
Lycium barbarum active glycopeptide
(LBAG) on melanoma. LBAG was extracted and isolated from the fruit of
Lycium barbarum using aqueous
alcoholic precipitation and identified using ultra-performance liquid chromatography-quadrupole-time of flightmass
spectrometry. Various assays including cell apoptosis, cell cycle analysis, colony formation assay, cell
scratch test, flow cytometry, and Western blot were performed to evaluate the effects of LBAG on melanoma.
Results: The results showed that LBAG has a molecular weight of 10-15 kDa and contains Man, Rha, GlcA, Glc,
Gal, and Ara18 amino acids. Treatment with LBAG significantly decreased B16 cell proliferation and induced
cell cycle arrest at the G0/G phase, accompanied by the accumulation of reactive oxygen species. Western blot
analysis revealed that the phosphorylation of P38-MAPK and AKT, as well as the expression of N-acetyl-Lcysteine,
were related to cell apoptosis and cell cycle regulation. In mouse xenografts, LBAG inhibited tumor
growth through the P38-MAPK and AKT signaling pathways.
Conclusion: In conclusion, the anti-melanoma activity of LBAG may induce apoptosis in cancer cells through
ROSmediated activation of the P38-MAPK and AKT signaling pathways. These findings provide a foundation
for further research on the anti-melanoma potential of LBAG.
