Title:Curcuminoids Modulated the IL-6/JAK/STAT3 Signaling Pathway in LoVo
and HT-29 Colorectal Cancer Cells
Volume: 29
Issue: 36
Author(s): Qian Li, Yanting Ding, Ying Ou, Manjing Li, Ponsiree Jithavech, Visarut Buranasudja, Boonchoo Sritularak, Yichun Xu*, Pornchai Rojsitthisak*Junsong Han*
Affiliation:
- National Engineering Research Center for Biochip at Shanghai, Shanghai Biochip Limited Corporation, Shanghai 201203, PR China
- Department of Pathology, Shanghai Tongji Hospital, Tongji Hospital Affiliated to Tongji University, Shanghai 200065, PR
China
- Center of Excellence in Natural Products for Ageing and Chronic Diseases, Chulalongkorn University, Bangkok 10330, Thailand
- Department of
Food and Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Chulalongkorn University, 254 Phayathai Road,
Pathumwan, Bangkok 10330, Thailand
- National Engineering Research Center for Biochip at Shanghai, Shanghai Biochip Limited Corporation, Shanghai 201203, PR China
Keywords:
Curcumin, desmethoxycurcumin, bisdesmethoxycurcumin, colorectal cancer, JAK/STAT3, colorectal cancer cells.
Abstract:
Background: Curcuminoids, including curcumin, desmethoxycurcumin, and bisdesmethoxycurcumin,
are natural polyphenolic compounds that exhibit various biological properties, such as antioxidant, anti-inflammatory,
and anticancer activities. Dysregulation of the interleukin (IL)-6-mediated Janus kinase/signal
transducer and activator of transcription 3 (JAK/STAT3) signaling pathway is closely associated with the development
of colorectal cancer (CRC).
Methods: Here, we have evaluated the modulation of the IL-6/JAK/STAT3 pathway of curcumin, desmethoxycurcumin,
and bisdesmethoxycurcumin in LoVo and HT-29 colorectal cancer cells with a single molecular array
(Simoa), western blot analysis, real-time polymerase chain reaction (PCR), and pathway analysis system.
Results: The study showed that curcuminoids suppressed the amount of IL-6 in LoVo and HT-29 colorectal
cancer cells. Meanwhile, curcuminoids inhibited the expression of inflammation regulator-related microRNA
(miRNA). We also found that the expression of total STAT3 was downregulated by curcuminoids. Moreover,
the pathway analysis system showed that curcuminoids inactivated the JAK/STAT3 signaling pathway. Taken
together, we demonstrated that the anti-cancer activities of curcuminoids against colorectal cancer are due to
the modulation of the IL-6/JAK/STAT3 cascade.
Conclusion: Curcuminoids could be a promising anti-cancer agent for the treatment of human colorectal cancer.
