Title:miR-628-5p is a Potential Novel Prognosis Biomarker, Associated with Immune
Infiltration in Bladder Urothelial Carcinoma
Volume: 29
Issue: 31
Author(s): Hong Huang, Qingchun Xu, Yonghai Zhang*, Yizhou Zhou, Kaiqun Ma and Yingxun Luo
Affiliation:
- Department of Urology, Shantou Central Hospital, Jinping District, Shantou 515031, China
Keywords:
microRNA-628-5p, bladder urothelial carcinoma (BLCA), prognosis, immune infiltration, gene expression, pathway, PI3K-Akt.
Abstract:
Background: microRNA-628-5p (miR-628-5p) has a significant impact on certain types of cancer.
The precise function of miR-628-5p in the context of bladder urothelial carcinoma (BLCA) remains ambiguous.
Objective: We aimed to investigate the role of miR-628-5p in BLCA.
Methods: The samples were collected from The Cancer Genome Atlas (TCGA). Statistics were employed to
evaluate the correlation and predictive significance of miR-628-5p. We analyzed the target genes and regulatory
network of miR-628-5p and the correlation between miR-628-5p and immune infiltration. The expression
of miR-628-5p in BLCA cells was confirmed by quantitative reverse-transcription PCR (qRT-PCR).
Results: miR-628-5p exhibited differential expression across various types of cancer. There was a significant
association between high expression of miR-628-5p and primary therapy outcome (p < 0.05). High expression
of miR-628-5p was observed to be associated with poorer overall survival (HR: 1.42; 95% CI: 1.06-1.90; p =
0.02), progress free survival (HR: 1.57; 95% CI: 1.17-2.11; p = 0.003), and disease specific survival (HR:
1.83; 95% CI: 1.28-2.62; p = 0.001) in BLCA. miR-628-5p was an independent prognostic factor in BLCA
and may be involved in the development of the disease through various pathways, including focal adhesion,
ECM-receptor interaction, PI3K-Akt signaling pathway, and MAPK signaling pathway, and among others.
miR-628-5p expression was significantly correlated with immune infiltration in BLCA patients. Compared to
normal bladder epithelial cells, BLCA cell lines exhibited a significant upregulation of miR-628-5p.
Conclusion: It is possible that miR-628-5p could serve as a hopeful therapeutic target and prognostic biomarker
for individuals with BLCA.
