Title:Integrating Network Pharmacology and Experimental Verification to Explore the
Targets and Mechanism for Panax Notoginseng Saponins against Coronary In-stent
Restenosis
Volume: 29
Issue: 28
Author(s): Yuanchao Li, Shenghan Gao, Hongying Zhu and Jianbo Wang*
Affiliation:
- Department of Interventional Radiology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of
Medicine, Shanghai 200233, China
Keywords:
Panax notoginseng saponins, coronary artery, in-stent restenosis, network pharmacology, molecular docking, molecular mechanism, vascular smooth muscle cell.
Abstract:
Background: Despite widespread application of drug-eluting stents in coronary intervention,
in-stent restenosis (ISR) is still a daunting complication in clinical practice. Panax notoginseng saponins
(PNS) are considered to be effective herb compounds for preventing ISR.
Objective: This study aimed to elucidate the targets and mechanisms of PNS in ISR prevention using network
pharmacology approaches and experimental verification.
Methods: Relevant targets of PNS active compounds were collected from the HERB database and PharmMapper.
The ISR-related targets were obtained from the GeneCards database and the Comparative Toxicogenomics
Database. The GO and KEGG enrichment analysis was performed using R software. The String
database and Cytoscape software were employed to build the PPI and compounds-targets-pathways-disease
networks. Finally, Molecular docking performed by Autodock Vina and cellular experiments were used to validate
network pharmacology results.
Results: There were 40 common targets between PNS targets and ISR targets. GO analysis revealed that these
targets focused on multiple ISR-related biological processes, including cell proliferation and migration, cell
adhesion, inflammatory response, and anti-thrombosis and so on. The KEGG enrichment results suggested
that PNS could regulate multiple signaling pathways to inhibit or delay the development and occurrence of
ISR. The molecular docking and cellular experiments results verified the network pharmacology results.
Conclusion: This study demonstrated that the potential molecular mechanisms of PNS for ISR prevention
involved multiple compounds, targets, and pathways. These findings provide a theoretical reference and
experimental basis for the clinical application and product development of PNS for the prevention of ISR.
