Title:Design and Evaluation of SLNs Encapsulated Curcumin-based Topical Formulation
for the Management of Cervical Cancer
Volume: 23
Issue: 16
Author(s): Manu Singhai, Vikas Pandey, Sumel Ashique, Ghanshyam Das Gupta, Daisy Arora, Tanweer Haider and Neeraj Mishra*
Affiliation:
- Amity Institute of Pharmacy, Amity University
Madhya Pradesh, Gwalior, Madhya Pradesh, India
Keywords:
Cervical cancer, design of experiment (DoE), box-behnken design (BBD), curcumin, chitosan, solid lipid nanoparticles.
Abstract:
Objective: Curcumin has the propensity to inhibit cancer growth, slow cancer development, increase chemotherapy
effectiveness, and shield healthy cells from radiation treatment harm. As a result of curcumin's ability to block
several signaling pathways, cervical cancer cells can once again proliferate normally. To optimize topically applied
curcumin-loaded solid lipid nanoparticles (SLNPs) for the treatment of cervical cancer, this study set out to establish
the relationship between design variables and experimental data. It also performed in vitro characterizations to determine
the formulation's efficacy and safety.
Methods: Curcumin-loaded SLNPs were constructed and optimized using a systematic design of experiment (DoE)
technique. SLNPs that were loaded with curcumin were produced utilizing a cold emulsification ultrasonication process.
Using the Box Behnken Design, it was determined how independent variables (factors) like the quantity of lipid
(A), the quantity of phospholipid (B), and the concentration of surfactant (C) affected the responses of the dependent
variables (responses), such as particle size (Y1), polydispersity index (PDI) (Y2), and entrapment efficiency (EE) (Y3)
(BBD).
Results: The ideal formulation (SLN9) was chosen using the desirability technique based on 3-D surface response
graphs. Using polynomial equations and three-dimensional surface plots, the influence of independent factors on the
dependent variables was evaluated. The observed responses were almost equal to the levels that the optimal formulation
expected. The improved SLNP gel's shape and other physicochemical characteristics were also assessed, and they
were determined to be ideal. The sustained release profile of the produced formulations was validated by in vitro release
tests. Studies on hemolysis, immunogenic response, and in vitro cell cytotoxicity demonstrate the efficacy and
safety of the formulations.
Conclusion: To improve the treatment effect, chitosan-coated SLNPs may carry encapsulated curcumin to the desired
location and facilitate its localization and deposition in the desired vaginal tissue.
