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摘要
基于主要凹槽侧三聚体形成寡核苷酸(TFOs)对目标双工DNA的序列特异性识别,抗原策略已成为一种备受关注的基因靶向工具。三联体DNA是通过特异性碱基三联体,由TFOs与目标双链DNA的同嘌呤链之间的Hoogsteen或反向Hoogsteen氢键相互作用形成的,从而导致已建立的序列特异性。然而,在靶同源嘌呤DNA序列的TA和CG碱基对中,被称为非天然核苷的反转位点可以与2 ' -脱氧胸苷(dT)和2 ' -脱氧胞苷(dC)形成令人满意的相互作用,这极大地限制了经典稳定碱基三联体甚至三联体DNA的形成。因此,设计能够有效识别CG或/和TA反转位点并具有满意选择性的非天然型核苷,对扩大三联体序列具有重要意义。在这里,本文主要综述了目前在三联体DNA形成过程中识别CG或/和TA反转位点的新型非天然核苷的研究进展,以对抗双链DNA。
关键词: 三联体形成寡核苷酸(TFO),三联体DNA,反转位点,非天然核苷酸,识别,抗原策略,脱氧胸腺嘧啶。
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