Title:The Effects of Trigonella Foenum-graecum L. on Post-surgical Adhesion
Band Formation
Volume: 21
Issue: 8
Author(s): Seyedeh Elnaz Nazari, Nima Khalili-Tanha, Shima Mehrabadi, Leila Mobasheri, Hamideh Naimi, Fatemeh khojasteh-Leylakoohi, Mohammad Dashtiahangar, Hamid Fiuji, Hassan Mottaghi Moghaddam Shahri, Sayyed Hadi Sayyed Hoseinian, Seyed Mahdi Hassanian, Hamid Reza Ghorbani, Mohsen Aliakbarian, Gordon A Ferns, Majid Khazaei*Amir Avan*
Affiliation:
- Department of Medical Physiology, Metabolic Syndrome Research Center, Mashhad University of Medical Sciences,
Mashhad, Iran
- Basic Medical Sciences Institute, Mashhad University of Medical Sciences, Mashhad, Iran
- Department of Medical Physiology, Metabolic Syndrome Research Center, Mashhad University of Medical Sciences,
Mashhad, Iran
- Basic Medical Sciences Institute, Mashhad University of Medical Sciences, Mashhad, Iran
- Medical Genetics Research Center,
Mashhad University of Medical Sciences, Mashhad, Iran
Keywords:
Trigonella Foenum-graecum L., fenugreek, intra-abdominal adhesion, inflammation, fibrosis, inflammatory cell infiltration.
Abstract: Background: Intra-abdominal adhesions are severe complications which occur after abdominal
surgery. Currently, no specific anti-adhesive medications can completely prevent Intra-abdominal adhesion
formation. Therefore, recent studies are exploring new approaches for preventing this complication.
Anti-inflammatory properties of Trigonella Foenum-graecum L. (Fenugreek) have been reported in various
studies. In this experiment, a murine model was used to evaluate the potential anti-adhesive activity
of Fenugreek in vivo.
Objective: This experiment aimed to examine the anti-adhesive activity of Fenugreek in the prevention of
postsurgical Intra-abdominal adhesions.
Methods: We have adhered to the ARRIVE guidelines during these experimental studies. After abdominal
surgery, for nine days, Fenugreek (400 mg/kg) was given by gavage to male Wistar rats (n = 6).
Following that, all animals were sacrificed to assess the anti-inflammatory and anti-fibrotic effects of
Fenugreek using Hematoxylin & eosin staining and Masson’s trichrome staining.
Results: Our results showed that Fenugreek hydro-alcoholic extract could significantly reduce the adhesion
band formation based on Nair and Leach Scoring system (P < 0.01). The histological assessment also
represented less inflammatory cell infiltration and less collagen deposition in the treatment group than in
the positive control group (P < 0.01).
Conclusion: This study showed that Fenugreek extract could attenuate post-surgical adhesion band formation
by inhibiting pathological responses (Inflammation and fibrosis) following surgery.
