Login Register Cart 0
Generic placeholder image

Letters in Drug Design & Discovery

Editor-in-Chief

ISSN (Print): 1570-1808
ISSN (Online): 1875-628X

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Assessment of Wound Healing Potential of Simvastatin Emulgel in Rat Excision Wound Model

Author(s): Surbhi Gupta, Anubhav Anand* and Ramesh Kumar Gupta

Volume 21, Issue 9, 2024

Published on: 03 May, 2023

Page: [1493 - 1501] Pages: 9

DOI: 10.2174/1570180820666230412091219

Price: $65

Abstract

Background: Microorganisms can infect wounds and disrupt healing by causing inflammation and tissue injury.

Objective: Simvastatin (SIM) is reported to have antibacterial activity; therefore, this research is designed to evaluate the efficacy of simvastatin emulgel in wound healing.

Methods: The simvastatin emulgel was assessed for physical appearance, rheological behaviour, spreadability, pH, ex vivo drug release and in vivo activity. The wound-healing activity was assessed by percent wound contraction, hydroxyproline estimation and histopathological evidence in wound-induced albino rats.

Results: The simvastatin emulgel exhibited viscosity and spreadability of 6875 ± 531.21 mPa.s and 10.15 ± 0.31 g.cm/sec, respectively. The simvastatin emulgel exhibited wound contraction in wound healing studies compared to the reference product (p < 0.05). This activity was statistically significant (p < 0.05) in comparison to the control. The hydroxyproline content supported the wound-healing promoting effect of simvastatin emulgel, and marked improvements in the skin histological architecture were seen in the histopathological study.

Conclusion: The study manifested the promising potential of simvastatin emulgel in speeding the healing of rat wounds with epithelization. Therefore, the designed simvastatin emulgel can be used as an option for the conventional topical dosage form.

Keywords: Simvastatin, emulgel, sesame oil, carbopol 934, tween 80, wound healing, hydroxyproline.

« Previous Next »
Graphical Abstract

[1]
Khan, A.R.; Riaz, M.; Bin Abdulhak, A.A.; Al-Tannir, M.A.; Garbati, M.A.; Erwin, P.J.; Baddour, L.M.; Tleyjeh, I.M. The role of statins in prevention and treatment of community acquired pneumonia: A systematic review and meta-analysis. PLoS One, 2013, 8(1), e52929.
[http://dx.doi.org/10.1371/journal.pone.0052929] [PMID: 23349694]
[2]
Björkhem-Bergman, L.; Bergman, P.; Andersson, J.; Lindh, J.D. Statin treatment and mortality in bacterial infections--a systematic review and meta-analysis. PLoS One, 2010, 5(5), e10702.
[http://dx.doi.org/10.1371/journal.pone.0010702] [PMID: 20502712]
[3]
Graziano, T.S.; Cuzzullin, M.C.; Franco, G.C.; Schwartz-Filho, H.O.; de Andrade, E.D.; Groppo, F.C.; Cogo-Müller, K. Statins and antimicrobial effects: Simvastatin as a potential drug against staphylococcus aureus biofilm. PLoS One, 2015, 10(5), e0128098.
[http://dx.doi.org/10.1371/journal.pone.0128098] [PMID: 26020797]
[4]
Jiang, T.; Han, N.; Zhao, B.; Xie, Y.; Wang, S. Enhanced dissolution rate and oral bioavailability of simvastatin nanocrystal prepared by sonoprecipitation. Drug Dev. Ind. Pharm., 2012, 38(10), 1230-1239.
[http://dx.doi.org/10.3109/03639045.2011.645830] [PMID: 22229827]
[5]
Ajazuddin, A.; Alexander, A.; Khichariya, A.; Gupta, S.; Patel, R.J.; Giri, T.K.; Tripathi, D.K. Recent expansions in an emergent novel drug delivery technology. Emulgel. J. Control. Release, 2013, 171(2), 122-132.
[http://dx.doi.org/10.1016/j.jconrel.2013.06.030] [PMID: 23831051]
[6]
Munir, A.; Ahmad, M.; Malik, M.Z.; Minhas, M.U. Analysis of simvastatin using a simple and fast high performance liquid chromatography-ultra violet method: Development, validation and application in solubility studies. Trop. J. Pharm. Res., 2014, 13(1), 135.
[http://dx.doi.org/10.4314/tjpr.v13i1.19]
[7]
Yadav, S.; Wairkar, S.; Invally, M.; Ranade, S.Y. Topical emulgel of tolnaftate with penetration enhancer: Development, characterization and antifungal activity. Indian J. Med Res. Pharm. Sci., 2017, 4, 28-35.
[8]
Sabu, K.R.; Basarkar, G.D. Formulation, development and in vitro evaluation of terbinafine hydrochloride emulgel for topical fungal infection. Int. J. Pharm. Sci. Rev. Res., 2013, 21, 168-173.
[9]
Jamadar, M.J.; Husen, S.R. Preparation and evaluation of herbal gel formulation. Indian J. Pharm. Educ. Res., 2017, 1, 201-224.
[10]
Sheetlani, J.; Singh Thakur, B. Preparation and enlargement of topical flurbiprofen emulgel by with xanthan gum. Int. J. Sci. Res. Engin. Technol., 2020, 01, 29-40.
[11]
Devesh, K.; Vyas, R.B.; Chaitali, L.; Manish, P. Formulation, development and characterization of emulgel of a NSAID’S. Pharm. Chem. J., 2014, 1, 9-16.
[12]
Ahmed, M.M.; Fatima, F.; Anwer, M.K.; Ibnouf, E.O.; Kalam, M.A.; Alshamsan, A.; Aldawsari, M.F.; Alalaiwe, A.; Ansari, M.J. Formulation and in vitro evaluation of topical nanosponge-based gel containing butenafine for the treatment of fungal skin infection. Saudi Pharm. J., 2021, 29(5), 467-477.
[http://dx.doi.org/10.1016/j.jsps.2021.04.010] [PMID: 34135673]
[13]
Pranali, S.; Charushila, S.; Sayali, C.; Namrata, M. Design and characterisation of emulgel of an antifungal drug. J. Pharm. Sci. Res., 2019, 11, 2357-2361.
[14]
Nagar, H.K.; Srivastava, A.K.; Srivastava, R.; Kurmi, M.L.; Chandel, H.S.; Ranawat, M.S. Pharmacological investigation of the wound healing activity of Cestrum nocturnum (L.) Ointment in wistar albino rats. J. Pharm. , 2016, 2016, 1-8.
[http://dx.doi.org/10.1155/2016/9249040] [PMID: 27018126]
[15]
Farahpour, M.R.; Dilmaghanian, A.; Faridy, M.; Karashi, E. Topical Moltkia coerulea hydroethanolic extract accelerates the repair of excision wound in a rat model. Chin. J. Traumatol., 2016, 19(2), 97-103.
[http://dx.doi.org/10.1016/j.cjtee.2015.08.005] [PMID: 27140217]
[16]
Ang, L.; Darwis, Y.; Koh, R.; Gah, L.K.; Yew, M.; Por, L.; Yam, M. Wound healing property of curcuminoids as a microcapsule-incorporated cream. Pharmaceutics, 2019, 11(5), 205.
[http://dx.doi.org/10.3390/pharmaceutics11050205] [PMID: 31052413]
[17]
Shailajan, S.; Menon, S.; Pednekar, S.; Singh, A. Wound healing efficacy of Jatyadi Taila: In vivo evaluation in rat using excision wound model. J. Ethnopharmacol., 2011, 138(1), 99-104.
[http://dx.doi.org/10.1016/j.jep.2011.08.050] [PMID: 21907784]
[18]
Jain, S.; Goswami, M.; Anil, B.; Arora, V. Skin irritation study of intradermal patch of chitosan containing trazodone-HCl on rat skin. Int. J. Res. Pharm. Biomed. Sci., 2011, 2, 1082-1084.
[19]
Baibhav, J.; Gurpreet, S. AC, R.; Seema, S. Development and characterization of clarithromycin emulgel for topical delivery. Int. J. Drug Dev. Res., 2012, 4, 310-323.
[20]
Thangamani, S.; Mohammad, H.; Abushahba, M.F.N.; Hamed, M.I. Exploring simvastatin, an antihyperlipidemic drug, as a potential topical antibacterial agent. Sci. Rep., 2015, 5, 16407.
[http://dx.doi.org/10.1038/srep16407] [PMID: 26553420]
[21]
Stojadinovic, O.; Lebrun, E.; Pastar, I.; Kirsner, R. Statins as potential therapeutic agents for healing disorders. Expert. Rev. Dermatol., 2010, 5, 689-698.
[http://dx.doi.org/10.1586/edm.10.60]
[22]
Ko, H.H.T.; Lareu, R.R.; Dix, B.R.; Hughes, J.D. In vitro antibacterial effects of statins against bacterial pathogens causing skin infections. Eur. J. Clin. Microbiol. Infect. Dis., 2018, 37(6), 1125-1135.
[http://dx.doi.org/10.1007/s10096-018-3227-5] [PMID: 29569046]
[23]
Wang, C.C.; Yang, P.W.; Yang, S.F.; Hsieh, K.P.; Tseng, S.P.; Lin, Y.C. Topical simvastatin promotes healing of Staphylococcus aureus -contaminated cutaneous wounds. Int. Wound J., 2016, 13(6), 1150-1157.
[http://dx.doi.org/10.1111/iwj.12431] [PMID: 25752328]
[24]
Bergman, P.; Linde, C.; Pütsep, K.; Pohanka, A.; Normark, S.; Henriques-Normark, B.; Andersson, J.; Björkhem-Bergman, L. Studies on the antibacterial effects of statins--in vitro and in vivo. PLoS One, 2011, 6(8), e24394.
[http://dx.doi.org/10.1371/journal.pone.0024394] [PMID: 21912631]

Rights & Permissions Print Cite
Article Metrics
12
1
© 2024 Bentham Science Publishers | Privacy Policy