Title:Antheraea proylei J. Sericin Induces Apoptosis in a Caspase-dependent Manner in A549 and HeLa Cells
Volume: 24
Issue: 9
Author(s): Potsangbam Jolly Devi, Asem Robinson Singh, Naorem Tarundas Singh, Laishram Rupachandra Singh, Sanjenbam Kunjeshwori Devi and Lisam Shanjukumar Singh*
Affiliation:
- Department of Biotechnology, Cancer and Molecular Biology Division, Manipur University, Canchipur Imphal, Manipur, 795003,
India
Keywords:
Sericin, Antheraea proylei J., silk cocoons, Caspase-3, PARP, MAPK.
Abstract:
Background: In spite of much progress in cancer, the global cancer burden is still significant and increasing.
Sericin, an adhesive protein of silk cocoons, has been shown to be a potential protein in various biomedical applications,
including cancer therapeutics. The present study evaluates the anticancer property of sericin from cocoons of
Antheraea proylei J (SAP) against human lung cancer (A549) and cervical cancer (HeLa) cell lines. This is the first
report of anti-cancer activity of the non-mulberry silkworm A. proylei J.
Objective: Establish the antiproliferative potential of SAP. 2. Identify the molecular mechanism of cell death induced
by SAP on two different cell lines.
Aims: To investigate the anticancer activity of sericin preparation from cocoons of A. proylei.
Methods: SAP was prepared from cocoons of A. proylei J. by the process of the degumming method. Cytotoxic activity
was assessed by MTT assay, and genotoxicity was assessed by comet assay. Cleavage of caspase and PARP proteins
and phosphorylation of MAPK pathway members were analysed by Western blotting. Cell cycle analysis was done by
flow cytometer.
Results: SAP causes cytotoxicity to A549 and HeLa cell lines with the IC50 values 3.8 and 3.9 μg/μl respectively. SAP
induces apoptosis in a dose-dependent manner through caspase-3 and p38, MAPK pathways in A549 and HeLa cells.
Moreover, in A549 and HeLa cells, SAP induces cell cycle arrest at the S phase in a dose-dependent manner.
Conclusion: The difference in the molecular mechanisms of apoptosis induced by SAP in A549 and HeLa cell lines
may be due to the difference in the genotypes of the cancer cell lines. However, further investigation is warranted. The
overall results of the present study envisage the possibility of using SAP as an anti-tumorigenic agent.
