The Use of MALDI-TOF Mass Spectrometry In Quantification of the Stability of Prolyl Endopeptidase Inhibitors

Title:The Use of MALDI-TOF Mass Spectrometry In Quantification of the Stability of Prolyl Endopeptidase Inhibitors

Volume: 3 Issue: 6

Author(s): Jorn Schmidt, Michael Wermann, Fred Rosche and Hans-Uirich Demuth*

Affiliation:

• Hans-Knoll-Institute of Natural Product Research e.V., Jena, Department of Drug Biochemistry, Weinbergweg 23, D-06120 Halle (Saale), Germany

Abstract: Z-Phe-Pro-(isonicotinic acid methylester) methylketone is a highly potent inhibitor (K_i^*= 18 nM) of prolyl endopeptidase from Flavobacterium meningosepticum. It acts as a slow-binding inhibitor and decomposes to compounds still possessing inhibitory activity.The pathway of decomposition was analyzed qualitatively and quantitatively applying MALDI-TOF mass spectrometry.

Cite this article as:

Schmidt Jorn, Wermann Michael, Rosche Fred and Demuth Hans-Uirich*, The Use of MALDI-TOF Mass Spectrometry In Quantification of the Stability of Prolyl Endopeptidase Inhibitors, Protein & Peptide Letters 1996; 3 (6) . https://dx.doi.org/10.2174/092986650306221101113916