Abstract
Z-Phe-Pro-(isonicotinic acid methylester) methylketone is a highly potent inhibitor (Ki*= 18 nM) of prolyl endopeptidase from Flavobacterium meningosepticum. It acts as a slow-binding inhibitor and decomposes to compounds still possessing inhibitory activity.The pathway of decomposition was analyzed qualitatively and quantitatively applying MALDI-TOF mass spectrometry.
Protein & Peptide Letters
Title:The Use of MALDI-TOF Mass Spectrometry In Quantification of the Stability of Prolyl Endopeptidase Inhibitors
Volume: 3 Issue: 6
Author(s): Jorn Schmidt, Michael Wermann, Fred Rosche and Hans-Uirich Demuth*
Affiliation:
- Hans-Knoll-Institute of Natural Product Research e.V., Jena, Department of Drug Biochemistry, Weinbergweg 23, D-06120 Halle (Saale), Germany
Abstract: Z-Phe-Pro-(isonicotinic acid methylester) methylketone is a highly potent inhibitor (Ki*= 18 nM) of prolyl endopeptidase from Flavobacterium meningosepticum. It acts as a slow-binding inhibitor and decomposes to compounds still possessing inhibitory activity.The pathway of decomposition was analyzed qualitatively and quantitatively applying MALDI-TOF mass spectrometry.
Export Options
About this article
Cite this article as:
Schmidt Jorn, Wermann Michael, Rosche Fred and Demuth Hans-Uirich*, The Use of MALDI-TOF Mass Spectrometry In Quantification of the Stability of Prolyl Endopeptidase Inhibitors, Protein & Peptide Letters 1996; 3 (6) . https://dx.doi.org/10.2174/092986650306221101113916
DOI https://dx.doi.org/10.2174/092986650306221101113916 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
![Wayfinder Image](/images/wayfinder.jpg)
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers