Abstract
For N-terminally fluoresceine labelled KLALKLALKALKAALKLA-NH2 (I) extensive incorporation into aortic endothelial cells (AEC) was found, resulting in an about 500-fold enrichment within the cell interior. The cellular uptake was not influenced by energy depletion and only partly by lowering the temperature to 0oC. Using double-D-amino acid analogs of this peptide, possessing a graduate propensity to adopt anα-helical structure, clear dependency of the peptide incorporation upon amphipathicity could be demonstrated.
Protein & Peptide Letters
Title:Nonendocytic, Amphipathcity Dependent Cellular Uptake of Helical Model Peptides
Volume: 3 Issue: 6
Author(s): Johannes Oehlke*, Eberhard Krause, Burkhard Wiesner, Michael Beyermann and Michael Bienert
Affiliation:
- Institute of Molecular Pharmacology, Alfred-Kowalke-Str. 4, D-10315 Berlin, Germany
Abstract: For N-terminally fluoresceine labelled KLALKLALKALKAALKLA-NH2 (I) extensive incorporation into aortic endothelial cells (AEC) was found, resulting in an about 500-fold enrichment within the cell interior. The cellular uptake was not influenced by energy depletion and only partly by lowering the temperature to 0oC. Using double-D-amino acid analogs of this peptide, possessing a graduate propensity to adopt anα-helical structure, clear dependency of the peptide incorporation upon amphipathicity could be demonstrated.
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Cite this article as:
Oehlke Johannes*, Krause Eberhard, Wiesner Burkhard, Beyermann Michael and Bienert Michael, Nonendocytic, Amphipathcity Dependent Cellular Uptake of Helical Model Peptides, Protein & Peptide Letters 1996; 3 (6) . https://dx.doi.org/10.2174/092986650306221101121141
DOI https://dx.doi.org/10.2174/092986650306221101121141 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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