Nonendocytic, Amphipathcity Dependent Cellular Uptake of Helical Model Peptides

Title:Nonendocytic, Amphipathcity Dependent Cellular Uptake of Helical Model Peptides

Volume: 3 Issue: 6

Author(s): Johannes Oehlke*, Eberhard Krause, Burkhard Wiesner, Michael Beyermann and Michael Bienert

Affiliation:

• Institute of Molecular Pharmacology, Alfred-Kowalke-Str. 4, D-10315 Berlin, Germany

Abstract: For N-terminally fluoresceine labelled KLALKLALKALKAALKLA-NH2 (I) extensive incorporation into aortic endothelial cells (AEC) was found, resulting in an about 500-fold enrichment within the cell interior. The cellular uptake was not influenced by energy depletion and only partly by lowering the temperature to 0^oC. Using double-D-amino acid analogs of this peptide, possessing a graduate propensity to adopt anα-helical structure, clear dependency of the peptide incorporation upon amphipathicity could be demonstrated.

Cite this article as:

Oehlke Johannes*, Krause Eberhard, Wiesner Burkhard, Beyermann Michael and Bienert Michael, Nonendocytic, Amphipathcity Dependent Cellular Uptake of Helical Model Peptides, Protein & Peptide Letters 1996; 3 (6) . https://dx.doi.org/10.2174/092986650306221101121141