Synthesis of Multiphosphorylated Peptide From a C-Terminal Bovine Rhodopsin Sequence

Title:Synthesis of Multiphosphorylated Peptide From a C-Terminal Bovine Rhodopsin Sequence

Volume: 3 Issue: 6

Author(s): Anatol Arendt*, J. Hugh McDowell, Galina Abdulaeva and Paul A. Hargrave

Affiliation:

• Department of Ophthalmology, University of Florida, Gainesville, FL 32610, USA

Abstract: We have synthesized and purified a 19-amino acid peptide, containing 4 phosphothreonines and 3 phosphoserines, of sequence derived from the C-terminal region of bovine rhodopsin. The peptide has been synthesized on Pamt resin using Boc-0-(diphenylphosphono)-serine and -threonine. Cleavage and deblocking have been performed in a single step using catalytic hydrogenolysis in the presence of palladium (II) and platinum (IV) with anhydrous trifluoroacetic acid as solvent.

Cite this article as:

Arendt Anatol*, McDowell Hugh J., Abdulaeva Galina and Hargrave A. Paul, Synthesis of Multiphosphorylated Peptide From a C-Terminal Bovine Rhodopsin Sequence, Protein & Peptide Letters 1996; 3 (6) . https://dx.doi.org/10.2174/092986650306221101110029