S-(1,2-Dicarboxyethyl)Glutathione, Intrinsic Tripeptide In Liver, Heart And Lens, Elevates γ-Glutamylcysteine Synthetase .Activity

Title:S-(1,2-Dicarboxyethyl)Glutathione, Intrinsic Tripeptide In Liver, Heart And Lens, Elevates γ-Glutamylcysteine Synthetase .Activity

Volume: 3 Issue: 6

Author(s): Naoko Nakagawa, Seiji Tsuboi, Naoki Kaneko, Takahiro Sakaue, Kazumi Ogata and Shinji Ohmori*

Affiliation:

• Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-Naka 1-1-1, Okayama 700, Japan

Abstract: The effect of S-(1,2-dicarboxyethyl)glutathione(DCE-GS) on glutathione (GSH) synthesis, especially γ-glutamylcysteine synthetase (γ-GCS), was studied in rat liver homogenate.It was found that the feedback inhibition of the γ-GCS by GSH was released by the addition of DCE-GS in the dose dependent manner. Namely, the activity of γ-GCS decreased to 17% of the normal level when GSH at the concentration of 10 mM was added to the reaction mixture. However, the addition of DCE-GS offset the inhibition at 20 μM and increased the γ-GCS activity to 1.7 and 3 times from the normal level at 50 and 100 μM, respectively .These results suggest that DCE-GS plays a role in stimulating the GSH synthesis.

Cite this article as:

Nakagawa Naoko, Tsuboi Seiji, Kaneko Naoki, Sakaue Takahiro, Ogata Kazumi and Ohmori Shinji*, S-(1,2-Dicarboxyethyl)Glutathione, Intrinsic Tripeptide In Liver, Heart And Lens, Elevates γ-Glutamylcysteine Synthetase .Activity, Protein & Peptide Letters 1996; 3 (6) . https://dx.doi.org/10.2174/092986650306221101103525