Title:ROS-mediated Genotoxicity and Apoptosis Induced by a Novel Salicylaldimine
Derivatives in Human Cervical Cancer Cells
Volume: 30
Issue: 33
关键词:
细胞凋亡，宫颈癌，化疗，细胞毒性，DNA损伤，配体L2b，氧化应激，水杨醛二胺。
摘要:
Background: Cervical cancer is one of the most common types of cancer
among women. Therefore, cancer studies are underway for a new chemo-agent with
more effect on cancer cells and fewer side effects on normal human healthy cells. The
currently studied novel ligand L2b as a reduced salicylaldimine derivative was examined
in seven cell lines, HeLa, DU-145, PC3, DLD-1, ECC, HT-29, and PNT1-A as a control.
Aim: Because of the antiproliferative ability of L2b, this study intends to look at the apoptotic,
cytotoxic, and genotoxic activity of L2b on HeLa.
Methods: For this purpose, MTT assay is for screening cytotoxic effects, comet assay for
looking for DNA damaging or genotoxicity levels, ELISA and DNA fragmentation for
apoptotic measuring, AO/EB stain test for checking the rates of live, apoptotic and necrotic
cells were performed. To reveal the oxidative state, OSI was assessed by total oxidant
and antioxidant status ratios. FRAP assay was calculated for ferric-reducing antioxidant
power, using total thiol and GSH assays to measure the antioxidant values of HeLa cells.
Results: Of this result, we have found a tremendous effect of L2b on HeLa cells, especially
in raising the ROS rate, damaging their DNA, and causing a range of reactions leading
to apoptosis.
Conclusion: In conclusion, the data predict which ligand L2b is capable of rising apoptosis
in vitro cervical cancer cell line studied. Further cancer studies are needed to reveal
the apoptosis pathways of the ligand L2b in the HeLa cell line and its anticancer drug potency
in vivo work.
