Title:Plant Alpha-amylase Inhibitors: Steady Kinetic Study, Bidens odorata
Aqueous Infusion Toxicity and Stability in Digestive Tract Simulation
Volume: 19
Issue: 1
Author(s): Roberto Arredondo Valdés*, José Luis Martínez Hernández, Sonia Yesenia Silva Belmares, Elda Patricia Segura Ceniceros*, Rodolfo Ramos González, Raihana Kunakova, Radik Zaynullin and Anna Iliná*
Affiliation:
- Department of Nanobioscience, Autonomous University of Coahuila, Coahuila, Mexico
- Department of Nanobioscience, Autonomous University of Coahuila, Coahuila, Mexico
- Department of Nanobioscience, Autonomous University of Coahuila, Coahuila, Mexico
Keywords:
α-amylase inhibition, ethanolic, watery medicinal plant, extracts, Bidens odorata toxicity, functional hypoglycemic food.
Abstract:
Background: α-Amylase inhibitors are considered an important therapeutic target to control
type 2 diabetes mellitus, reducing postprandial hyperglycemia. Medicinal plants are an important source
with inhibitory activities of this enzyme but are little studied.
Objective: The present study explored the α-amylase inhibition with extracts of 11 medicinal plants
available in Saltillo, Mexico; the kinetic mechanism of inhibition of selected extracts and their phytochemical
screening; evaluation of the toxicity of Bidens odorata extract in Artemia salina model, as
well as estimation of its inhibitory effect under in vitro digestive tract conditions.
Methods: The inhibitory assays were carried out spectrophotometrically with aqueous suspensions of
the extracts obtained after evaporation of solvent from aqueous and ethanolic infusions.
Results: Eleven plants showed an inhibitory effect of α-amylase above 10% of the initial activity at
666.7 ppm. Four plants were selected for kinetic assay due to the inhibitory effect near or higher than
20%. The IC50 for the aqueous suspension of the ethanolic extract of Bidens odorata was 851 ppm, similar
to that detected with the drug acarbose. The inhibition mechanism for Bidens odorata, Cinchona
succirub, and Opuntia ficus-indicata was competitive, and for Cnidoscolus chayamansa it was uncompetitive.
All selected extracts presented flavonoids, the majority contained terpenoids, 3 contained tannins
and phenols. The aqueous infusion of Bidens odorata - model of a functional drink showed no toxicity
and was characterized by resistance for 60 min to the simulated stomach and intestinal conditions
in vitro.
Conclusion: The findings of this study revealed the species of medicinal plants, which were not previously
considered as sources of α-amylase inhibitors, and their kinetic mechanisms of inhibition, which
can be used for functional hypoglycemic food preparation.
