Title:Antioxidant, α-amylase and α-glucosidase Inhibitory Activities of Aqueous
and Methanolic Extracts From Athamanta sicula L.
Volume: 19
Issue: 2
Author(s): Karima Loucif*, Fatima Benchikh, Chawki Ben Souici and Smain Amira
Affiliation:
- Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of
Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria
Keywords:
Athamanta sicula L., phenolic content, antioxidant properties, α-amylase inhibitory activity, and α-glucosidase inhibitory activity, methanolic extracts.
Abstract:
Introduction: Oxidative stress plays a major role in developing diabetes complications;
therefore, it is possible to use natural antioxidants as therapeutic agents for diabetes.
Aims: This study aimed to find an important source of phenolics from Athamanta sicula L. (A. sicula)
and confirm that this plant could be a significant source of medically important natural compounds by
confirming its antioxidant, α-amylase and α-glucosidase inhibitory potential.
Methods: Antioxidant property was performed using 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical
scavenging, metal chelating, and cupric reducing antioxidant capacity methods. The α-amylase and α-
glucosidase inhibitory activities were investigated using an in vitro model. Moreover, polyphenols and
flavonoid contents of the tested extracts were carried out.
Results: The methanolic extract exhibited the highest phenolic and flavonoid content compared to the
aqueous extract. However, aqueous extract possessed the strongest antioxidant activity (DPPH scavenging,
metal chelating, and Cupric ion reducing antioxidant capacities), α-amylase and α-glucosidase inhibitory
effects. No correlation between phenolic content and antioxidant and enzyme inhibitory activities
of A. sicula L. extracts was observed.
Conclusion: The results showed that this plant could be a significant source of medically important natural
compounds.