Title:Non-surgical In Vivo Germ Cell-mediated Gene Editing by CRISPR
Mutagenic Chain Reaction with the Aid of Magnetic Nanoparticles
Volume: 30
Issue: 7
Author(s): Maryam Vahdat Lasemi and Amirhossein Sahebkar*
Affiliation:
- Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
- Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
- School of Medicine, The University of Western Australia, Perth, Australia
- Department of Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Keywords:
Gene editing, CRISPR, nanoparticles, germ cell, electroporation , magnetic nanoparticles.
Abstract: Gene therapy via germline cells leads to a permanent genetic modification.
The promise of this method is due to its potential for providing a stable therapeutic effect
for all who inherit the gene of interest. If germinal therapy is successfully performed, it
can eliminate certain diseases from the family and the population. The feasibility of genetic
modification in the human germline raises several controversial and bioethical issues.
However, gene transfer via male and female germinal cells has been recently explored
in animal models. Previous studies have shown that delivering DNA to the testes
followed by electroporation is relatively successful in producing germline-mediated alterations.
Since this method includes surgical procedures, non-surgical, safer, and less timeconsuming
methods would be ideal. Herein, we discuss a potential approach for nonsurgical
in vivo germ cell-mediated gene editing by CRISPR mutagenic chain reaction with
the aid of magnetic nanoparticles.
