Title:Ethyl Pyruvate Alleviating Inflammatory Response after Diabetic Cerebral
Hemorrhage
Volume: 19
Issue: 2
Author(s): Yueying Wang, Ke Li, Zhiyi Liu, Yulan Sun, JiaJun Wang, Qi Liu, Yuejia Song*Jiping Qi*
Affiliation:
- Department of
Endocrinology, First Clinical Hospital, Harbin Medical University, Harbin 150001, China
- Department of Pathology, First Clinical Hospital, Harbin Medical University, Harbin 150001, China
Keywords:
Diabetic cerebral hemorrhage, EP, inflammasome, HMGB1, TLR4, ethyl pyruvate.
Abstract:
Objective: This study’s purpose is to investigate the neuroprotective role of ethyl pyruvate
(EP) in the pathogenesis of diabetic intracerebral hemorrhage.
Methods: The present study used a mouse model of collagenase-induced intracerebral hemorrhage
(ICH) and streptozotocin-induced diabetes. The C57BL/6 mice were randomly divided into 3
groups: sham operation, diabetic cerebral hemorrhage, and diabetic cerebral hemorrhage with EP.
The EP (80 mg/kg) and EP (50 mg/kg) were injected intraperitoneally one day and one hour before
modeling. The protein expression levels of high mobility group box 1 (HMGB1) and NOD-like
receptors 3 (NLRP3) were detected with western blot. The mRNA levels of HMGB1 and toll-like
receptor 4 (TLR4) were measured by quantitative real-time polymerase chain reaction (PCR). Immunofluorescence
and ELISA were performed to confirm some inflammatory factors.
Results: Compared to the normal diabetic intracerebral hemorrhage group, the mRNA and protein
expression levels of HMGB1 and TLR4 were downregulated in the EP-affected group with diabetic
cerebral hemorrhage, together with the downregulation of the expression of inflammasomes, including
NLRP3, apoptosis-associated speck-like protein containing CARD (ASC), and caspase 1.
Conclusion: EP can reduce the inflammatory response after diabetic intracerebral hemorrhage and
may inhibit the activation of inflammasomes by the HMGB1/TLR4 pathway.