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Infectious Disorders - Drug Targets

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ISSN (Print): 1871-5265
ISSN (Online): 2212-3989

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Research Article

The Effect of Curcumin on the Expression of INFγ, TNF-α, and iNOS Genes in PBMCs Infected with Leishmania major [MRHO/IR/75/ER]

Author(s): Soheila Alinejad, Shahram Khademvatan*, Shahla Amani*, Negar Asadi, Khosrow Hazrati Tappeh, Elham Yousefi and Touraj Miandoabi

Volume 22, Issue 6, 2022

Published on: 15 June, 2022

Article ID: e040422203031 Pages: 7

DOI: 10.2174/1871526522666220404083220

Price: $65

Abstract

Background: Leishmaniasis, caused by the Leishmania parasite, is one of the most important tropical neglected diseases. The urgent search for effective, inexpensive, and preferably herbal anti-leishmanial agents, is needed.

Objective: Curcumin is a natural polyphenolic compound derived from turmeric that is well known for its antioxidant, anti-inflammatory, anti-tumor, and anti-cancer activity.

Methods: The present work evaluates the anti-leishmanial [Leishmania major] activity of curcumin. The infected PBMCs were treated with curcumin. The ROS level at 6, 12, 24 h and gene expression levels at 24, 48, and 72 h of PBMCs after treatment with curcumin were determined.

Results: Based on the results, the curcumin concentrations of 268 μM [24 h] and 181.2 μM [72 h] were defined as IC50 against L. major promastigotes. Treatment of L. major infected-peripheral blood mononuclear cells [PBMCs] with IC50 concentrations of curcumin, depending on exposure time, significantly induced the reactive oxygen species [ROS] generation and increased the expression levels of interferongamma [IFN-γ], tumor necrosis factor-alpha [TNF-α], and nitric oxide synthase [iNOS] genes.

Conclusion: These findings suggest the potential of curcumin against Leishmaniasis.

Keywords: Curcumin, gene expression, Leishmania major, PBMCs, tumor necrosis factor, interferon gamma.

Graphical Abstract

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