Title:Comparative Study to Characterise the Pharmaceutical Potential of
Synthesised Snake Venom Bradykinin-Potentiating Peptides In Vivo
Volume: 29
Issue: 42
关键词:
蛇毒缓激肽增强肽、赖诺普利、肌酐、曲细精管、肝脏组织形态测定、ACE。
摘要:
Background: Bradykinin-potentiating peptides (BPPs) are snake venom peptides
inhibiting the angiotensin-converting enzyme (ACE). ACE plays an important role
in the regulation of blood pressure. BPPs lead to the development of ACE inhibitors for
the treatment of hypertension.
Objective: The objective of the present work was to carry out a comprehensive comparative
study of four synthesised snake venom BPPs in vivo.
Methods: Four synthesised snake venom BPPs were administered to rats via the intraperitoneal
route for 15 days at a fixed dose. Lisinopril was used as a comparative standard.
Thirty male albino rats were divided into six groups: A, B, C, D, E (lisinopril), and F
(control). Group F was maintained as the control group and given only saline. After 15
days, blood samples and tissues were removed for the study of selective biochemical parameters
and histomorphometric analysis. Statistical evaluation of all results was also performed.
Results: The results indicated that peptide I, with the sequence ZSAPGNEAIPP, was
highly toxic and adversely affected all the biochemical and histological parameters
studied in this work. Peptide II (ZNWPHPQIPP) and peptide IV (ZQWAQGRAPHPP)
showed lower toxicity. None of the BPPs raised the serum creatinine level and exhibited
nephroprotective effects. Although lisinopril raised the creatinine level, it showed a protective
role towards the pancreas and lungs in parallel.
Conclusion: The present work shows that although there is a high sequence similarity between
the four BPPs, their in vivo activity varies. The sequences of peptide II and peptide
IV can be used to improve the design of current ACE inhibitors used for hypertension
treatment.
