Title:miR-27b-3p is Highly Expressed in Serum of Patients with Preeclampsia
and has Clinical Significance
Volume: 22
Issue: 6
Author(s): Yang Yang, Fang Tang and Xuezhi Zhao*
Affiliation:
- Department of Gynecology,
Hospital of Obstetrics and Gynecology Affiliated to Medical College of Zhejiang University, Hangzhou City, Zhejiang
310000, China
Keywords:
Preeclampsia, miR-27b-3p, PPARG, receiver operating characteristic curve, pearson's analysis, mild preeclampsia, severe preeclampsia, serum.
Abstract:
Background: Preeclampsia (PE) is defined as a salient complication of late pregnancy.
microRNAs (miRNAs) have emerged as critical biological regulators in PE. This study determined
miR-27b-3p expression in serum of PE patients and investigated its clinical significance in PE.
Methods: This study enrolled a total of 130 pregnant women, including 90 PE patients (51 mild PE
and 39 severe PE) and 40 healthy controls. miR-27b-3p expression in the serum of PE patients and
healthy controls was detected using RT-qPCR. The correlation among miR-27b-3p expression and
24-h urine protein, systolic blood pressure (SBP), diastolic blood pressure (DBP), serum creatinine,
and fetal birth weight was analyzed using Pearson's correlation coefficient. The targeting relationship
between miR-27b-3p and PPARG was verified. PPARG protein level in PE patients was
detected using ELISA kits. The predictive efficiency of miR-27b-3p and PPARG in PE was analyzed
using the receiver operating characteristic (ROC) curve.
Results: Compared to normal pregnant women, PE pregnant women, especially severe PE patients,
had higher miR-27b-3p expression. miR-27b-3p was positively correlated with 24-h urine protein,
SBP, DBP, and serum creatinine but negatively correlated with fetal birth weight. PPARG was
poorly expressed in PE patients and negatively correlated with miR-27b-3p. ROC curve showed
that both miR-27b-3p and PPARG had good predictive efficacy on PE.
Conclusion: miR-27b-3p expression in serum of pregnant women with PE was positively correlated
with the severity of PE symptoms, suggesting the involvement of miR-27b-3p in PE occurrence.
