Title:Anti-oxidant and Anticancerous Effect of Fomitopsis officinalis (Vill. ex Fr. Bond. et
Sing) Mushroom on Hepatocellular Carcinoma Cells In Vitro through NF-kB Pathway
Volume: 22
Issue: 8
Author(s): Nyamsambuu Altannavch, Xi Zhou, Md. Asaduzzaman Khan, Ashfaque Ahmed, Shinen Naranmandakh, Jun-Jiang Fu and Han-Chun Chen*
Affiliation:
- Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, 172 Tongzipo Road, Changsha,
Hunan 410013, China
Keywords:
Fomitopsis officinalis, antioxidant activity, apoptosis, anticancer, HepG2, NF-kB signaling pathway.
Abstract:
Background: Fomitopsis officinalis (Vill. ex Fr. Bond. et Sing) is a medicinal mushroom, commonly called
‘Agarikon’; it has traditionally been used to treat cough and asthma in the Mongolian population.
Objective: The objective of the study was to examine the significance of biological activity of F. officinalis and evaluation
of the antioxidant activity and anticancer activity of six fractions of F. officinalis residues (Fo1-powder form dissolved
in ethanol, Fo2-petroleum ether residue, Fo3-chloroformic, Fo4-ethylacetate, Fo5-buthanolic, and Fo6-waterethanolic)
against hepatocellular carcinoma cells.
Methods: We performed in vitro studies of cell proliferation and viability assay, annexin V-FITC/Propidium Iodide
assay, and NF-kB signaling pathway by immunoblot analysis.
Results: Our findings revealed that all six fractions/extracts have antioxidant activity, and somehow, they exert anticancerous
effects against cancer cells. In cancerous cell lines (HepG2 and LO2), Fo3 chloroformic extract promoted
the cancer cell apoptosis and cell viability, activated G2/M-phase cell cycle, and selectively induced NF-kB proteins,
revealing as a novel antitumor extract.
Conclusion: This study reports that Fo3-chloroformic extract is rich in antitumor activity, which was previously not
investigated in cancer. To develop the impact of F. officinalis among natural products to treat/prevent oxidative stress
disorders or cancers, further examinations of F. officinalis are needed to develop new natural drugs to treat cancer.
However, this study assessed only one extract, Fo3-chloroformic, which has a significant impact against cancer cell
lines.