Title:Antimutagenic, Antiproliferative and Antioxidant Properties of Sea Grape Leaf Extract Fractions (Coccoloba uvifera L.)
Volume: 21
Issue: 16
Author(s): Jorge A. Ramos-Hernández, Montserrat Calderón-Santoyo, Armando Burgos-Hernández, Joel S. García-Romo, Arturo Navarro-Ocaña, María G. Burboa-Zazueta, Edgar Sandoval-Petris and Juan A. Ragazzo-Sánchez*
Affiliation:
- Laboratorio Integral de Investigacion en Alimentos, Tecnologico Nacional de Mexico-Instituto Tecnologico de Tepic, Av. Tecnologico 2595, C.P. 63175 Tepic, Nayarit,Mexico
Keywords:
Coccoloba uvifera leaf, hexane extract, fractions, antimutagenicity, antioxidant, antiproliferation.
Abstract:
Background: Cancer is a disease characterized by the invasion and uncontrolled growth of cells.
One of the best ways to minimize the harmful effects of mutagens is through the use of natural antimutagens. In
this regard, the search for new antimutagens that act in the chemoprevention could represent a promising field
in this area.
Objective: In this study biological potential of 11 fractions from Coccoloba uvifera L. leaf hexane extract was
evaluated by several in vitro tests.
Methods: Leaves were lyophilized and hexane extraction was performed. The extract was fractionated by column
chromatography with hexane, ethyl acetate, and methanol. The antimutagenic (Ames test), antiproliferative
(MTT test), and antioxidant capacity (DPPH, ABTS, and ferrous ion chelation) of the fractions were evaluated.
Results: Fractions 4, 6, 8, and 9 have antimutagenic activity (against sodium azide in strain TA100), fraction 11
showed antiproliferative capacity (IC50 of 24 ± 9 μg/mL in cells of HCT 116). The fractions with the highest activity
were analyzed by HPLC-MS and lupeol, acacetin, and β-sitosterol were identified.
Conclusion: This study demonstrates, for the first time, the bioactivity of C. uvifera leaf as a new source of
High Biological Value Compounds (HBVC), which can be of interest to the food and pharmaceutical industries.
