Title:Recent Advances in Glycosidase Probes Used in Escherichia Coli Detection
Volume: 28
Issue: 26
Author(s): Xianhu Wei, Ying Feng, Minling Chen, Shuhong Zhang, Moutong Chen, Jumei Zhang, Youxiong Zhang, Qinghua Ye, Yu Ding, Liang Xue and Qingping Wu*
Affiliation:
- Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070,China
Keywords:
Bacterial pathogens, Glycosidase substrates, Chromogenic substrates, Fluorogenic substrates, Luminogenic
substrates, Electrochemical substrates.
Abstract: Pathogenic Escherichia coli poses a serious threat to global public health and
is especially dangerous with the increase of antibiotic resistance. β-Glucuronidase (GUS)
and some other glycosidases can serve as useful biomarkers or indicators for the detection
of E. coli. The probes made up of a glycosyl residue (recognition group), a label or
reporter group, and a linkage that is generally a direct glycosidic bond, are powerful analytical
tools. Upon hydrolysis of the glycosidic linkages by the corresponding glycosidases,
these probes irreversibly release detectable labels or reporter molecules. A variety of
such glycosidase probes have been developed and applied for the detection of E. coli or
the development of various corresponding detection methods. This paper provides an
overview of recent advances in this field, covering the development and applications of
chromogenic, fluorogenic, luminogenic, and electrochemical glycosidase substrates. The
challenges and opportunities in the probe development for detection of E. coli are also
discussed.
