Title:Bioactive Peptides Originating from Gastrointestinal Endogenous Proteins in the Growing Pig: In Vivo Identification
Volume: 27
Issue: 11
Author(s): Lakshmi A. Dave, Maria Hayes, Leticia Mora, Shane M. Rutherfurd, Carlos A. Montoya and Paul J. Moughan*
Affiliation:
- Riddet Institute, Massey University, Private Bag 11222, Palmerston North 4442,New Zealand
Keywords:
Angiotensin-I converting enzyme (ACE-I) inhibition, antioxidant peptides, exogenous bioactive peptides, ferric reducing antioxidant
power, gut non-dietary proteins, microsomal lipid peroxidation inhibition, non-dietary proteins, porcine model, serum albumin.
Abstract:
Background: Recent in silico and in vitro studies have shown that gastrointestinal endogenous proteins
(GEP) are a source of bioactive peptides. To date, however, the presence of such peptides in the lumen of
the digestive tract has not been demonstrated.
Objective: We investigated the generation of GEP-derived bioactive peptides in the growing pig fed a proteinfree
diet.
Methods: Stomach chyme (SC) and jejunal digesta (JD) fractions from 6 growing pigs (two sampling times)
were assessed for their angiotensin-I-converting enzyme (ACE-I; EC 3.4.15.1) inhibition, and antioxidant activity
using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition, ferric reducing antioxidant power (FRAP) and
microsomal lipid peroxidation (MLP) inhibition assays.
Results: Two of the fractions prepared from JD samples inhibited ACE-I and DPPH by 81 (± 2.80)% and 94
(±0.66)%. SC fractions were found to inhibit MLP between 15-39 (±3.52-1.40)%. The study identified over 180
novel peptide sequences that were related to the determined bioactivities, including a porcine serum albuminderived
peptide (FAKTCVADESAENCDKS), corresponding to f(7-23) of the human serum albumin peptide
LVNEVTEFAKTCVADESAENCDKSLHTLF that was previously identified from the digests of the latter GEP.
Conclusion: This study provides the first in vivo evidence for GEP as a source of bioactive peptides. These new
findings help advance our knowledge of the latent bioactive role of GEP-derived peptides in mammalian nutrition
and health and their potential pharmaceutical applications.