Title:Harnessing Phage Display for the Discovery of Peptide-Based Drugs and Monoclonal Antibodies
Volume: 28
Issue: 40
Author(s): Yuyang Li, Min Liu and Songbo Xie*
Affiliation:
- College of Life Sciences, Shandong Provincial Key Laboratory of Animal Resistance Biology, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Institute of Biomedical Sciences, Shandong Normal University, Jinan, Shandong 250014,China
Keywords:
Phage display, peptide-based drug, monoclonal antibody, drug discovery and development, microfluidics,
PROTAC.
Abstract: Phage display is a powerful high-throughput screening technology that presents
a large and diverse selection of functional peptides, proteins, or antibody fragments
on phage capsid surfaces for affinity determination towards a target of interest. Due to its
advantages of a large screening capacity, mass production through fermentation, and
straightforward execution, phage display has been widely used in bioengineering and
biomedicine, especially for diagnostics and therapeutics. With the advent of next-generation
sequencing and microfluidics technologies, phage display has become an even more
powerful and popular tool for drug discovery and development. Here, we briefly review
phage display technology and its application to drug discovery, including the discovery
and development of peptide-based drugs and monoclonal antibodies. Combined with the
emerging proteolysis targeting chimeras (PROTAC), we also discuss the advantages and
future directions, aiming to facilitate drug discovery and development.
