Title:Far Infrared Technology (FIT) Therapy Patches, Protects from Inflammation, Oxidative Stress and Promotes Cellular Vitality
Volume: 26
Issue: 34
Author(s): Donatella Pastore, Francesca Pacifici, Giampaolo Ciao, Valentina Bedin, Guido Pasquantonio and David Della-Morte*
Affiliation:
- Department of Systems Medicine, University of Rome Tor Vergata, Rome,Italy
Keywords:
Infrared technology, patches, ROS, inflammation, oxidative stress, chronic diseases.
Abstract:
Background: It is known from the most recent literature that far-infrared (FIR) radiations promote a
broad spectrum of therapeutic benefits for cells and tissues.
Objective: To identify molecular mechanisms by which FIT patches, as a far infrared technology, protects against
damage caused by inflammatory process and oxidative stress.
Methods: Endothelial cells (HUVEC, Human Umbilical Vein Endothelial Cells) were used as in vitro experimental
model. HUVEC were stimulated with a pro-inflammatory cytokine, TNF-α, or hydrogen peroxide (H2O2) to
induce oxidative stress. As markers of inflammation were evaluated: VCAM1 (Vascular Cell Adhesion Molecule
1), ICAM1 (Intercellular Adhesion Molecule 1) and E-Selectin by Western Blot analysis. Oxidative stress was
assessed by cytofluorimetric analysis. The experiments were performed on control cells (no patch) or in cells
treated with the FIT infrared technology applied on the basis of the culture plate.
Results: HUVEC stimulated with TNF-α and treated with FIT patches had significant reduction of the expression
of VCAM1, ICAM1 and E-Selectin (p<0.05). HUVEC stimulated with H2O2 and treated with FIT patches were
significantly protected from oxidative stress (p <0.01) when compared to control cells. We measured cell viability
and proliferation in HUVEC and HEK-293 (Human embryonic kidney cells) cells by MTT assay. HEK-293 and
HUVEC treated with FIT patches showed a significantly higher percentage of basal vitality compared to control
cells (p<0.0001 for HEK-293, p<0.05 for HUVEC).
Conclusion: FIT therapy patches - infrared technology, through these protective mechanisms, could be used in all
pathologies where an increase in inflammation, oxidative stress and degenerative state are present.