Title:Association of Interleukin-1 and Inteleukin-1 Receptor Antagonist Gene Polymorphisms with Multiple Sclerosis in Azeri Population of Iran
Volume: 20
Issue: 7
Author(s): Mohammad Asgharzadeh, Zahra A. Jigheh, Hossein S. Kafil, Mehdi Farhoudi, Daryoush S. Oskouei, Fatemeh Khaki-Khatibi, Manouchehr Fadaee , Ali Vegari, Vahid Asgharzadeh, Nima Najafi-Ghalelou, Behroz M. Poor and Jalil Rashedi*
Affiliation:
- Department of Laboratory Sciences, Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz,Iran
Keywords:
IL-1A, IL-1B, IL-1RA, polymorphism, multiple sclerosis, Iran.
Abstract:
Background: Cytokines as important mediators have a critical role in appropriate immune
responses, the irregular production of which can lead to Multiple Sclerosis (MS). Proinflammatory
cytokine interleukin-1 (IL-1) triggers inflammatory responses. Function and production of the cytokine
are influenced by IL-1 coding gene polymorphism and those antagonists gene polymorphism.
Objective: The aim of the present study was to evaluate the possible correlation between MS and IL-1
related alleles in Azeri population of Iran.
Methods: Variable number tandem repeats (VNTR) genotypes of 150 MS patients and 220 healthy
non-relative controls were determined.
Results: In the healthy controls, genotype TT at IL-1A (-889) location was significantly higher than
the MS patients (p=0.0001). However, a significant difference was not found between the two groups
in genotypic/allelic frequency at IL- 1B+ 3953 location. Evaluation of the IL-1RA gene revealed that
genotype 1/2, and genotype 1/3 were significantly higher in the healthy controls and MS patients, respectively.
Our findings indicated that the consumption of fast-food in MS patients was significantly
higher than controls (p= <0.05). Also, a considerable number of MS patients had inappropriate dieting
behaviors such as not eating breakfast (p= 0.0001), and irregular eating habits (p= 0.0001).
Conclusion: Polymorphisms of the IL-1B genes and common alleles of IL-1RA were not considered
as risk factors for MS disease. However, genotype TT at IL-1A (-889) location and the rare allele of
IL-1RA3 can be a potential risk factor for the disease. Furthermore, inappropriate dieting behaviors
and consumption of fast-food can increase the risk of MS.