Title:Anticancer Potential of Michelia champaca Linn. Bark Against Ehrlich Ascites Carcinoma (EAC) Cells in Swiss Albino Mice
Volume: 11
Issue: 1
Author(s): Ruksana Yesmin, Plabon Kumar Das, Hazrat Belal, Suraiya Aktar, Mst. Ayesha Siddika, Saharia Yeasmin Asha, Farjana Habib, Md. Abdur Rakib and Farhadul Islam*
Affiliation:
- Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6250,Bangladesh
Keywords:
Michelia champaca, chemotherapy, natural products, apoptosis, antioxidant, EAC cells.
Abstract:
Background: Adverse side effects of currently available therapies against cancer, leads
scientists to find effective compounds from natural sources.
Objective: In the present study, stem-bark of Mycelia champaca is subjected to evaluate its antiproliferative
effect against Ehrlich Ascites Carcinoma (EAC) cells. To date, the anti-proliferative effects
of M. champaca bark extract against EAC cell line has not been reported elsewhere. Therefore,
we intended to investigate the anti-proliferative potential of M. champaca bark extract against EAC
cells in vivo.
Methods: In vivo anticancer activity was evaluated against EAC cells bearing Swiss albino mice by
monitoring parameters such as tumor cell proliferation, tumor weight measurement, and survival
time, etc. The mechanism of EAC killing was examined by observation of cell morphology and
analysis the expression of certain cancer-related genes. In vitro antioxidant potentiality was determined
in terms of several common antioxidant assays. In addition, total phenolic and flavonoids
contents were measured to ensure the presence of phytochemicals.
Results: M. champaca bark extract showed strong antioxidant activities which were found to be
strongly correlated (P<0.001) with phenolics and flavonoids contents. Furthermore, it was found that
bark extract decreased tumor cell proliferation (77.46%; P<0.01), tumor weight (42.13%; P<0.001)
and increased life span of tumor-bearing mice (71.97%; P<0.01) at the dose of 250mg/kg (intraperitoneal;
i.p.). M. champaca bark also altered the depleted hematological parameters such as red blood
cell, white blood cell, hemoglobin (Hb%) towards normal in tumor bearing mice. In addition, upregulation
of p53, Bax and downregulation of Bcl-2 followed by treatment indicated M. champaca bark
could induce apoptosis of EAC cells.
Conclusion: These results indicated that MEMCB possesses significant cytotoxic activities against
EAC cells and has a strong in vitro antioxidant capacity. Therefore, the bark of M. champaca could
be considered as a potential resource of anti-cancer agents, which might be used to formulate effective
anticancer drugs.
